Related Experiment Video
Updated: May 21, 2026

Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast
Published on: May 15, 2018
Improvement of the growth defect in salt- and ethanol-tolerant yeast mutagenized with error-prone DNA polymerization
Kazukiyo Hayashi1, Shuntaro Yano, Hiroko Abe
1Division of Applied Life Science, Graduate School of Applied and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531, Japan.
Abstract:
Salt- and ethanol-tolerant mutants of Saccharomyces cerevisiae, isolated from the uracil-requiring mutant derived from Taiken No. 396 by proofreading-deficient DNA polymerization, showed less growth than their parent strain. The fusants, between these tolerant mutants and the lysine-requiring mutant from Taiken No. 396 obtained by the protoplast fusion, indicated improved growth.

