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Updated: May 21, 2026
A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
Published on: April 11, 2014
Covalent immobilization of ascorbate oxidase onto polycarbonate strip for L-ascorbic acid detection
Dileep Kumar Kannoujia1, Saroj Kumar, Pradip Nahar
1Institute of Genomics and Integrative Biology-CSIR, Mall Road, Delhi 110007, India.
Abstract:
Herein, a simple and rapid method is described for detection of L-ascorbic acid by ascorbate oxidase immobilized onto polycarbonate strip pre-activated by 1-fluoro-2-nitro-4-azidobenzene in photochemical reaction. Covalent attachment of ascorbate oxidase was confirmed by XPS studies. The immobilized-ascorbate oxidase shows higher pH, thermal and storage stability in comparison to free enzyme.
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