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Cell Culture

Cell Culture

Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media

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Related Experiment Video

Updated: May 21, 2026

Sandwich-like Microenvironments to Harness Cell/Material Interactions

Sandwich-like Microenvironments to Harness Cell/Material Interactions

Published on: August 4, 2015

Material strategies for creating artificial cell-instructive niches.

Faramarz Edalat¹, Iris Sheu, Sam Manoucheri

¹Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA.

Current Opinion in Biotechnology

|June 19, 2012

Summary

This summary is machine-generated.

Advanced biomaterials are revolutionizing tissue engineering by mimicking the body's natural cellular environment. These scaffolds incorporate structural, mechanical, and biochemical cues for enhanced cell interaction and tissue regeneration.

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Last Updated: May 21, 2026

Sandwich-like Microenvironments to Harness Cell/Material Interactions

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Published on: August 4, 2015

Fabrication of a Multiplexed Artificial Cellular MicroEnvironment Array

Fabrication of a Multiplexed Artificial Cellular MicroEnvironment Array

Published on: September 7, 2018

Manipulating Living Cells to Construct Stable 3D Cellular Assembly Without Artificial Scaffold

Manipulating Living Cells to Construct Stable 3D Cellular Assembly Without Artificial Scaffold

Published on: October 26, 2018

Area of Science:

Biomaterials Science
Tissue Engineering
Cellular Biology

Background:

Biomaterials are increasingly used as cellular scaffolds in tissue engineering.
Advanced strategies aim to create biomaterials that interact with cells and the in vivo environment in a biologically specific manner.

Purpose of the Study:

To present advanced material strategies for tissue engineering scaffolds.
To highlight methods for mimicking the extracellular matrix and in vivo cellular environment.

Main Methods:

Use of composite materials and advanced processing methods.
Integration of mechanical and topographical properties in scaffold design.
Incorporation of biochemical cues (cytokines) in various forms (tethered, soluble, time-released).
Replication of dynamic forces and biochemical gradients using microfluidics.

Main Results:

Strategies effectively mimic the extracellular matrix.
Scaffold design integrates mechanical and topographical features.
Biochemical cues are successfully incorporated.
Microfluidics enables replication of dynamic in vivo conditions.

Conclusions:

Advanced biomaterial strategies offer promising approaches for tissue engineering.
Mimicking the complex in vivo cellular environment is crucial for scaffold design.
Integration of multiple material properties and dynamic simulation enhances potential applications.