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Published on: January 21, 2020
A modified method for inducing periodontitis in dogs using a silk-wire twisted ligature
Se Eun Kim1, Eui Ri Lee, Yesran Lee
1Department of Veterinary Surgery, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
Journal of Veterinary Science
|June 19, 2012
Summary
A novel twisted silk and wire ligature (SWL) effectively induced advanced periodontitis in dogs. This method significantly worsened periodontal parameters and bone loss, proving useful for canine periodontitis research.
Area of Science:
- Periodontology
- Veterinary Dentistry
- Biomaterials Science
Background:
- Periodontitis is a complex inflammatory disease affecting tooth-supporting structures.
- Developing reliable animal models is crucial for understanding periodontitis pathogenesis and testing therapies.
- Current methods for inducing experimental periodontitis may have limitations in mimicking human disease progression.
Purpose of the Study:
- To evaluate the efficacy of a modified silk ligature twisted with wire (SWL) in inducing advanced experimental periodontitis in a canine model.
- To compare the effectiveness of the SWL method against other ligature materials (wire ligature - WL, silk ligature - SL) and a control group.
- To assess the impact of the SWL method on key periodontal indices and alveolar bone levels.
Main Methods:
- Twenty healthy dogs were used, with periodontitis induced in premolars and molars over 60 days.
- Dogs were divided into four groups: soft food only, WL, SL, and SWL.
- Periodontal indices (plaque, gingival, attachment level, bleeding) and dental radiographs were assessed before and after the 60-day period.
Main Results:
- The SWL group exhibited significantly exacerbated periodontal parameters, including plaque index, gingival index, clinical attachment level, and bleeding on probing, compared to other groups (p < 0.05).
- Radiographic evaluation revealed significantly lower alveolar bone levels in the SWL group by day 60 (p < 0.05).
- The silk ligature (SL) group showed a significantly shorter duration of ligature maintenance compared to the SWL group (p < 0.05).
Conclusions:
- The modified silk ligature twisted with wire (SWL) is an effective method for inducing advanced experimental periodontitis in canine models.
- The SWL method leads to significant clinical and radiographic signs of periodontitis, closely mimicking human disease progression.
- This canine model utilizing SWL provides a robust platform for future research into periodontitis mechanisms and therapeutic interventions.
