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Updated: May 21, 2026

Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun
Published on: April 18, 2014
'Shotgun DNA synthesis' for the high-throughput construction of large DNA molecules
Hwangbeom Kim1, Hyojun Han, Jinwoo Ahn
1Department of Chemistry, Yonsei University, Shinchon 134, Seoul 120749, Korea.
Abstract:
We developed a highly scalable 'shotgun' DNA synthesis technology by utilizing microchip oligonucleotides, shotgun assembly and next-generation sequencing technology. A pool of microchip oligonucleotides targeting a penicillin biosynthetic gene cluster were assembled into numerous random fragments, and tagged with 20 bp degenerate barcode primer pairs. An optimal set of error-free fragments were identified by high-throughput DNA sequencing, selectively amplified using the barcode sequences, and successfully assembled into the target gene cluster.
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