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Updated: May 21, 2026

Moving Upwards: A Simple and Flexible In Vitro Three-dimensional Invasion Assay Protocol

Moving Upwards: A Simple and Flexible In Vitro Three-dimensional Invasion Assay Protocol

Published on: March 12, 2018

A unique 3D in vitro cellular invasion assay.

Daniela F Quail¹, Tamara J Maciel, Kem Rogers

¹Western University, London, Ontario, Canada.

Journal of Biomolecular Screening

|June 19, 2012

Summary

This summary is machine-generated.

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This study introduces a novel 3D invasion assay for breast cancer, enabling quantifiable drug and microenvironment effect assessments. The assay mimics in vivo conditions, offering a more physiologically relevant in vitro model for studying cellular invasion.

Area of Science:

Oncology
Biotechnology
Cell Biology

Background:

Three-dimensional (3D) cell cultures and bioreactors are used for breast cancer cell co-culture.
Previous studies noted differences in invasion through collagen but lacked quantifiable methodology.
Existing in vitro assays often omit tumor 3D heterogeneity and multidirectional cell migration.

Purpose of the Study:

To develop a quantifiable 3D invasion assay for breast cancer.
To assess the impact of drugs and microenvironments on cancer cell invasion.
To create a physiologically relevant in vitro model recapitulating in vivo milieu.

Main Methods:

Utilized established 3D cell culture techniques in a bioreactor.
Developed a 3D invasion assay using cell clusters.

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Last Updated: May 21, 2026

Moving Upwards: A Simple and Flexible In Vitro Three-dimensional Invasion Assay Protocol

Moving Upwards: A Simple and Flexible In Vitro Three-dimensional Invasion Assay Protocol

Published on: March 12, 2018

Modeling and Imaging 3-Dimensional Collective Cell Invasion

Modeling and Imaging 3-Dimensional Collective Cell Invasion

Published on: December 7, 2011

Alternative Strategy to Analyze In Vitro Cell Invasion of 3D Cultures

Alternative Strategy to Analyze In Vitro Cell Invasion of 3D Cultures

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Implemented a novel whole-mount technique for fluorescence-based immunolocalization without sectioning.

Main Results:

The developed 3D invasion assay allows for the assessment of drug and microenvironment effects.
The whole-mount technique enables protein localization throughout the entire tumorsphere.
The assay provides a simple, inexpensive, and physiologically relevant context for studying cellular invasion.

Conclusions:

A novel 3D invasion assay has been developed for breast cancer research.
This assay offers a more accurate in vitro model for studying cellular invasion.
The methodology facilitates the evaluation of therapeutic interventions and microenvironmental factors in cancer progression.