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[Electron microscopic observations on Pseudomonas cocovenenans]
1National Institute for the Control of Pharmaceutical and Biological Products, Beijing.
Abstract:
A strain of food-poisoning bacterium has been isolated by Jin Jiexiang (1963) in China from the fermented cornflour that has gone bad. These pathogenic microorganism has been identified and named Pseudomonas by Zhao Naixin in 1988, which is the same species as P. cocovenenans. The characteristics of them were conformed to these of the species P. cepacia of section 2 of the genus Pseudomonas. In view of the fact that the fine structures of the above mentioned three strains of Pseudomonas have not been described yet, we decided to observe them with electron microscope. Results indicate there are many things in common among the three strains, such as: appearing short rods, 0.6-0.8 microns in diameter by 1.5-2.0 microns in length, one polar multiflagella; non-pili, non-capsules, non-endospores; containing intranuclear inclusions (electron-dense bodies or concentric laminae bodies), accumulating intracytoplasmic PHB granules; forming filaments, minicells and bizarrecells; producing extracellular cellulose-like materials by the three strains have not been reported previously.
Insights
Researchers studied three strains of food-poisoning Pseudomonas bacteria, identifying common fine structures like rods, multiflagella, and unique inclusions. This electron microscope study reveals previously unreported characteristics of these pathogenic microorganisms.
Area of Science:
- Microbiology
- Bacteriology
Context:
- Food-borne illness outbreaks linked to contaminated fermented foods.
- Identification of pathogenic microorganisms is crucial for public health.
Purpose:
- To describe the fine structures of three specific strains of Pseudomonas bacteria using electron microscopy.
- To compare and contrast the ultrastructural characteristics of these food-poisoning bacteria.
Summary:
- Three strains of food-poisoning Pseudomonas bacteria, including P. cocovenenans and P. cepacia, were examined via electron microscopy.
- Common ultrastructural features observed include short rods, polar multiflagella, intranuclear inclusions, intracytoplasmic PHB granules, and the formation of filaments, minicells, and bizarre cells.
- Previously unreported characteristics include the production of extracellular cellulose-like materials by these strains.
Impact:
- Provides detailed ultrastructural data for the taxonomic classification of these Pseudomonas species.
- Contributes to understanding the morphology of food-borne pathogens, potentially aiding in detection and control strategies.
- Highlights novel extracellular material production, opening avenues for further research into bacterial biopolymers.