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Updated: May 21, 2026
Multi-timescale Microscopy Methods for the Characterization of Fluorescently-labeled Microbubbles for Ultrasound-Triggered Drug Release
Published on: June 12, 2021
Real-time assessment of ultrasound-mediated drug delivery using fibered confocal fluorescence microscopy
Marc Derieppe1, Anna Yudina, Matthieu Lepetit-Coiffé
1Laboratory for Molecular and Functional Imaging: From Physiology to Therapy, FRE 3313-CNRS and University Bordeaux Segalen, 146, rue Léo Saignat, Case 117, 33076, Bordeaux cedex, France.
Purpose:
Transport across the plasma membrane is a critical step of drug delivery for weakly permeable compounds with intracellular mode of action. The purpose of this study is to demonstrate real-time monitoring of ultrasound (US)-mediated cell-impermeable model drug uptake with fibered confocal fluorescence microscopy (FCFM).
Procedures:
An in vitro setup was designed to combine a mono-element US transducer, a cell chamber with a monolayer of tumor cells together with SonoVue microbubbles, and a FCFM system. The cell-impermeable intercalating dye, SYTOX Green, was used to monitor US-mediated uptake.
Results:
The majority of the cell population showed fluorescence signal enhancement 10 s after US onset. The mean rate constant k of signal enhancement was calculated to be 0.23 ± 0.04 min(-1).
Conclusions:
Feasibility of real-time monitoring of US-mediated intracellular delivery by FCFM has been demonstrated. The method allowed quantitative assessment of model drug uptake, holding great promise for further local drug delivery studies.
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