Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
Rab Proteins01:14

Rab Proteins

Rab proteins constitute the largest family of monomeric GTPases, of which 70 members are present in humans. Rab proteins and their effectors regulate consecutive stages of vesicle transport such as vesicle transport, docking, and fusion to the correct recipient membrane.
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
CRISPR and crRNAs02:53

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Face/off: phase-specific modeling of lineage plasticity using near-patient models in genitourinary cancers.

Gene·2026
Same author

Game of clones: decipher lineage plasticity in hormone-driven cancers.

Cellular and molecular life sciences : CMLS·2026
Same author

Systemic Treatments in Men with De Novo Metastatic Prostate Cancer.

The Urologic clinics of North America·2026
Same author

Moving the Needle in Managing Men with Advanced Prostate Cancer.

The Urologic clinics of North America·2026
Same author

HOX code-based stratification reveals RUNX1T1-HDAC reprogramming as a targetable driver of lineage plasticity across cancers.

Cancer letters·2026
Same author

Navigating the winding road toward precision prostate cancer care.

Gene·2025

Related Experiment Video

Updated: May 21, 2026

Bioluminescence Resonance Energy Transfer (BRET)-Based Assay for Measuring Interactions of CRAF with 14-3-3 Proteins in Live Cells
06:44

Bioluminescence Resonance Energy Transfer (BRET)-Based Assay for Measuring Interactions of CRAF with 14-3-3 Proteins in Live Cells

Published on: March 1, 2024

CREBZF, a novel Smad8-binding protein.

Jae-Ho Lee1, Geun Taek Lee, Seok Joo Kwon

  • 1Section of Urologic Oncology and the Dean and Betty Gallo Prostate Cancer Center, The Cancer Institute of New Jersey and Robert Wood Johnson Medical School, New Brunswick, NJ 08903, USA.

Molecular and Cellular Biochemistry
|June 19, 2012
PubMed
Summary

Researchers identified CREBZF, a novel protein that binds to Smads 1, 5, and 8. This interaction inhibits bone morphogenetic protein (BMP) signaling, offering new insights into BMP pathway regulation.

More Related Videos

Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells
09:16

Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells

Published on: September 1, 2019

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
07:46

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Published on: December 11, 2020

Related Experiment Videos

Last Updated: May 21, 2026

Bioluminescence Resonance Energy Transfer (BRET)-Based Assay for Measuring Interactions of CRAF with 14-3-3 Proteins in Live Cells
06:44

Bioluminescence Resonance Energy Transfer (BRET)-Based Assay for Measuring Interactions of CRAF with 14-3-3 Proteins in Live Cells

Published on: March 1, 2024

Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells
09:16

Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells

Published on: September 1, 2019

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
07:46

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Published on: December 11, 2020

Area of Science:

  • Molecular Biology
  • Cell Signaling
  • Transcription Factors

Background:

  • Smad proteins act as key intracellular signal transducers in the transforming growth factor-beta (TGF-β) superfamily pathways.
  • While Smads 2/3 are well-characterized in TGF-β signaling, the roles and regulation of Smads 1, 5, and 8 in bone morphogenetic protein (BMP) pathways are less understood.
  • Understanding BMP-mediated Smad regulation is crucial for deciphering developmental and disease processes.

Purpose of the Study:

  • To identify novel proteins that interact with BMP-mediating Smads (Smads 1, 5, and 8).
  • To elucidate the functional consequences of these interactions on BMP signaling.
  • To investigate the role of CREBZF in the modulation of BMP pathways.

Main Methods:

  • Yeast two-hybrid screening using the Smad8 Mad homology 2 (MH2) domain as bait.
  • Co-immunoprecipitation assays to confirm protein interactions in a human prostate cancer cell line.
  • Reporter gene assays to assess the effect of CREBZF on BMP response element activity.
  • Cell proliferation assays to evaluate the impact of BMP-6 and CREBZF on cell growth.

Main Results:

  • A novel Smad-binding protein, CREBZF (cAMP response element-binding zinc finger), was identified.
  • The interaction between CREBZF and Smads 1, 5, and 8 was validated.
  • Overexpression of CREBZF suppressed BMP response element promoter activity.
  • CREBZF expression abrogated BMP-6-induced growth inhibition, indicating functional antagonism.

Conclusions:

  • CREBZF acts as an inhibitor of BMP-6 signaling by interacting with Smads 1, 5, and 8.
  • This discovery reveals a new regulatory mechanism within the BMP signaling pathway.
  • The identification of CREBZF contributes to a deeper understanding of BMP pathway modulation and its potential implications in diseases like cancer.