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Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)
Published on: May 20, 2013
Identification of the unknown transformation products derived from lincomycin using LC-HRMS technique
P Calza1, C Medana, E Padovano
1Dipartimento di Chimica, via P. Giuria 5, 10125 Torino. paola.calza@unito.it
Journal of Mass Spectrometry : JMS
|June 19, 2012
Summary
This study tracked lincomycin's environmental fate using high-resolution mass spectrometry. Researchers identified 21 transformation products, revealing hydroxylation as the primary degradation pathway for this antibiotic in aquatic environments.
Area of Science:
- Environmental Chemistry
- Analytical Chemistry
- Pharmaceutical Science
Background:
- Antibiotics like lincomycin can persist in aquatic environments.
- Understanding pharmaceutical transformation is crucial for environmental risk assessment.
Purpose of the Study:
- To comprehensively study the fate and transformation pathways of lincomycin in aquatic environments.
- To identify and characterize lincomycin transformation products (TPs) under various environmental conditions.
Main Methods:
- Utilized high-performance liquid chromatography-high resolution mass spectrometry (LC-HRMS) for precise identification of TPs.
- Employed MS(2) and MS(3) fragmentation patterns to characterize structures and differentiate isomers.
- Simulated aquatic phototransformation using dark, homogeneous photolysis, and heterogeneous photocatalysis (TiO2).
Main Results:
- Identified 21 main lincomycin transformation products.
- Characterized isomeric species through detailed MS(n) spectral analysis.
- Determined that hydroxylation (N-alkyl side chain or pyrrolidine moiety) is the major transformation process, alongside oxidation/reduction, demethylation, and pyranose ring cleavage.
Conclusions:
- Elucidated the primary transformation pathways of lincomycin in aquatic environments.
- Demonstrated the utility of LC-HRMS for identifying and characterizing pharmaceutical TPs.
- Provided insights into the environmental degradation of lincomycin, essential for ecological safety assessments.
