Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intranasal dopamine: Anatomical pathways, biological mechanisms and neuromodulatory potential.

Neuroscience and biobehavioral reviews·2026
Same author

Specificity and exon target space of splicing modifying compounds.

Nature communications·2026
Same author

Unfolding prion misfolding and the challenge of identifying effective therapeutics: is there hope on the horizon?

Expert opinion on drug discovery·2026
Same author

Use of deep learning to predict chronic wasting disease status based on animal movement.

Movement ecology·2026
Same author

Exploring PrP<sup>C</sup> unfolding as a critical step preceding its refolding in the context of PrP<sup>Sc</sup> propagation.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Quantifying Oxidized Methionines with Mass Spectrometry to Map the Surface of Hamster Prion Strains Sc237 and 139H.

Journal of the American Society for Mass Spectrometry·2026

Related Experiment Video

Updated: May 21, 2026

Metabolomic Analysis of Rat Brain by High Resolution Nuclear Magnetic Resonance Spectroscopy of Tissue Extracts
09:01

Metabolomic Analysis of Rat Brain by High Resolution Nuclear Magnetic Resonance Spectroscopy of Tissue Extracts

Published on: September 21, 2014

Human and rat brain lipofuscin proteome.

Philipp Ottis1, Katharina Koppe, Bruce Onisko

  • 1Department of Neuropathology, Heinrich Heine University of Düsseldorf, Düsseldorf, Germany.

Proteomics
|June 19, 2012
PubMed
Summary

Brain aging involves lipofuscin accumulation. This study identifies key proteins in human and rat brain lipofuscin, revealing common cellular pathways linked to aging and disease.

More Related Videos

Shotgun Lipidomics of Rodent Tissues
11:46

Shotgun Lipidomics of Rodent Tissues

Published on: November 18, 2022

Improved Lipofuscin Models and Quantification of Outer Segment Phagocytosis Capacity in Highly Polarized Human Retinal Pigment Epithelial Cultures
10:39

Improved Lipofuscin Models and Quantification of Outer Segment Phagocytosis Capacity in Highly Polarized Human Retinal Pigment Epithelial Cultures

Published on: April 14, 2023

Related Experiment Videos

Last Updated: May 21, 2026

Metabolomic Analysis of Rat Brain by High Resolution Nuclear Magnetic Resonance Spectroscopy of Tissue Extracts
09:01

Metabolomic Analysis of Rat Brain by High Resolution Nuclear Magnetic Resonance Spectroscopy of Tissue Extracts

Published on: September 21, 2014

Shotgun Lipidomics of Rodent Tissues
11:46

Shotgun Lipidomics of Rodent Tissues

Published on: November 18, 2022

Improved Lipofuscin Models and Quantification of Outer Segment Phagocytosis Capacity in Highly Polarized Human Retinal Pigment Epithelial Cultures
10:39

Improved Lipofuscin Models and Quantification of Outer Segment Phagocytosis Capacity in Highly Polarized Human Retinal Pigment Epithelial Cultures

Published on: April 14, 2023

Area of Science:

  • Neuroscience
  • Cellular Biology
  • Biochemistry

Background:

  • Lipofuscin, an autofluorescent pigment, accumulates in neurons and is a hallmark of brain aging.
  • Previously considered waste, lipofuscin's role in cellular pathology is increasingly suspected due to links with neuronal ceroid lipofuscinosis and macular degeneration.

Purpose of the Study:

  • To characterize the protein composition of human and rat brain lipofuscin.
  • To identify common molecular pathways involved in age-associated lipofuscin accumulation across species.

Main Methods:

  • Purification of autofluorescent material (lipofuscin) from human temporal and hippocampal cortices using two-step ultracentrifugation.
  • Proteomic analysis of purified human and rat brain lipofuscin.
  • Interspecies comparison of lipofuscin proteomes.

Main Results:

  • Identified a common set of 49 proteins in human brain lipofuscin, primarily from mitochondria, cytoskeleton, and cell membrane.
  • Validated findings in rat brain lipofuscin, revealing a 64% overlap in identified proteins.
  • Demonstrated high homology in brain lipofuscin proteomes between humans and rats despite significant lifespan differences.

Conclusions:

  • Human and rat brain lipofuscins share significant protein homology, suggesting conserved cellular pathomechanisms in age-associated accumulation.
  • The identified protein sets provide a foundation for investigating molecular pathways in dysfunctional lysosomal degradation during aging.