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Related Concept Videos

Voltammetric Techniques: Cyclic Voltammetry01:10

Voltammetric Techniques: Cyclic Voltammetry

Cyclic voltammetry (CV) is an electrochemical technique used to investigate the redox properties of a chemical species. It involves measuring the current response of an electrochemical cell as a function of the applied potential. The setup for cyclic voltammetry typically consists of a working electrode, a reference electrode, and a counter electrode—all immersed in an electrolyte solution. The working electrode is where the redox reaction of interest occurs, while the reference electrode...
Voltammetric Techniques: Linear-Scan (E vs Time)01:12

Voltammetric Techniques: Linear-Scan (E vs Time)

Polarography is a classical voltammetric technique used to analyze electrochemical reactions. This method applies a linear potential sweep to a dropping mercury electrode (DME), and the resulting current is measured. A dropping mercury electrode is commonly used as the working electrode in polarography. It consists of a capillary tube filled with mercury, where the tiny droplet forms at the tip. This droplet continuously drops from the capillary, creating a new electrode surface for each...
Voltammetric Techniques: Pulse Voltammetry01:17

Voltammetric Techniques: Pulse Voltammetry

Differential-pulse voltammetry (DPV) is a type of voltammetry that involves applying a series of voltage pulses to an electrochemical cell while measuring the resulting current. In DPV, the differential pulse or small potential pulses are superimposed on a linear potential sweep. The magnitude of these pulses is typically small, often in the millivolt range. Each voltage pulse lasts a short duration, usually in the order of a few milliseconds, and is applied at regular intervals along the...
Voltammetry: Stripping Methods01:13

Voltammetry: Stripping Methods

Anodic Stripping Voltammetry (ASV), Cathodic Stripping Voltammetry (CSV), and Adsorptive Stripping Voltammetry (AdSV) are electrochemical techniques used to determine trace amounts of analytes in solution. These methods involve applying a potential to an electrode and measuring the resulting current.
Anodic Stripping Voltammetry (ASV)
ASV is used to determine metals and metalloids at trace levels. It involves two steps: deposition and stripping. First, a negative potential is applied to the...
Voltammetry: Overview01:20

Voltammetry: Overview

Voltammetry is an electroanalytical technique in which the current flowing through an electrochemical cell is measured as a function of applied potential, typically under conditions of concentration polarization. The technique provides valuable information about redox-active species, and the current response is plotted as a voltammogram.
A voltammetric cell uses three electrodes: a working electrode, a reference electrode, and an auxiliary electrode. The redox reactions occur in the working...
Voltammograms: Overview01:16

Voltammograms: Overview

Voltammograms are current plots as a function of applied potential, offering insights into electrochemical systems. The shape of a voltammogram depends on how the current is measured and whether convection (heat transfer by fluid movement) is present or absent.
Shapes of Voltammograms

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Related Experiment Video

Updated: May 21, 2026

Modeling Fast-scan Cyclic Voltammetry Data from Electrically Stimulated Dopamine Neurotransmission Data Using QNsim1.0
07:41

Modeling Fast-scan Cyclic Voltammetry Data from Electrically Stimulated Dopamine Neurotransmission Data Using QNsim1.0

Published on: June 5, 2017

Optimizing the Temporal Resolution of Fast-Scan Cyclic Voltammetry.

Brian M Kile1, Paul L Walsh, Zoé A McElligott

  • 1Department of Chemistry and Neuroscience Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599.

ACS Chemical Neuroscience
|June 19, 2012
PubMed
Summary
This summary is machine-generated.

Fast-scan cyclic voltammetry (FSCV) at 60 Hz improves temporal resolution for monitoring dopamine release and uptake by the dopamine transporter (DAT). This enhanced FSCV method more accurately tracks rapid neuronal signaling compared to slower 10 Hz FSCV.

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Combined Infusion and Stimulation with Fast-Scan Cyclic Voltammetry (CIS-FSCV) to Assess Ventral Tegmental Area Receptor Regulation of Phasic Dopamine
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Combined Infusion and Stimulation with Fast-Scan Cyclic Voltammetry (CIS-FSCV) to Assess Ventral Tegmental Area Receptor Regulation of Phasic Dopamine

Published on: April 23, 2020

Related Experiment Videos

Last Updated: May 21, 2026

Modeling Fast-scan Cyclic Voltammetry Data from Electrically Stimulated Dopamine Neurotransmission Data Using QNsim1.0
07:41

Modeling Fast-scan Cyclic Voltammetry Data from Electrically Stimulated Dopamine Neurotransmission Data Using QNsim1.0

Published on: June 5, 2017

Combined Infusion and Stimulation with Fast-Scan Cyclic Voltammetry (CIS-FSCV) to Assess Ventral Tegmental Area Receptor Regulation of Phasic Dopamine
06:40

Combined Infusion and Stimulation with Fast-Scan Cyclic Voltammetry (CIS-FSCV) to Assess Ventral Tegmental Area Receptor Regulation of Phasic Dopamine

Published on: April 23, 2020

Area of Science:

  • Neuroscience
  • Electrochemistry
  • Analytical Chemistry

Background:

  • Carbon-fiber microelectrodes are used for electrochemical detection of dopamine release and uptake.
  • Constant potential amperometry (CPA) offers real-time monitoring but lacks chemical identification and calibration ease.
  • Fast-scan cyclic voltammetry (FSCV) provides species identification but suffers from delayed response times due to electrode adsorption.

Purpose of the Study:

  • To enhance the temporal resolution of FSCV for dopamine monitoring.
  • To improve FSCV's response time to be comparable with CPA.
  • To accurately measure dopamine transporter (DAT) activity in real-time.

Main Methods:

  • Increased FSCV waveform repetition rate from 10 Hz to 60 Hz using uncoated carbon-fiber electrodes.
  • Compared FSCV (10 Hz and 60 Hz) with CPA for dopamine uptake measurements.
  • Utilized transgenic mice overexpressing DAT and anesthetized rats for method validation.

Main Results:

  • Faster 60 Hz FSCV acquisition reduced time delays, aligning better with CPA measurements.
  • 10 Hz FSCV underestimated dopamine uptake by approximately 18%; 60 Hz FSCV provided comparable results to CPA.
  • 60 Hz FSCV accurately monitored increased DAT activity in transgenic mice and in vivo rat models.

Conclusions:

  • Increasing FSCV repetition rate to 60 Hz significantly improves temporal resolution for dopamine transporter studies.
  • High-speed FSCV offers a more accurate and chemically identifiable alternative to CPA for monitoring rapid neurotransmitter dynamics.
  • This optimized FSCV technique is valuable for studying neuronal dopamine signaling and transporter function in various models.