Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Blood Studies for Cardiovascular System III: Serum Lipid Profile

Understanding serum lipids is crucial for maintaining cardiovascular health and preventing heart disease and stroke.
Serum lipids are fats and fatty substances in the blood and are crucial for various bodily functions, including energy storage, cellular structure, and hormone production. Serum lipids consist of cholesterol, triglycerides, and phospholipids.
Cholesterol is a soft, fat-like substance found in all body cells. It is crucial for producing hormones, vitamin D, and substances that aid...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Potentially Atherogenic Combined Biochemical and Physiological Changes in Human Antiorthostatic Hypokinesia.

Bulletin of experimental biology and medicine·2026

Same author

In Vitro Study of Cancer Stem Cells and Reprogrammed CD3<sup>+</sup>CD8<sup>+</sup> T Cells: the Search for Personalized Diagnostics and Targeted Treatment of Small Cell Lung Cancer (Experience of a Pilot Study).

Bulletin of experimental biology and medicine·2025

Same author

Activation of the Complement System and Changes in the Content of Modified Lipoproteins in Antiorthostatic Hypokinesia in Humans and Mice.

Bulletin of experimental biology and medicine·2025

Same author

A Model of Combination Therapy of Lewis Lung Carcinoma using Resection and Cytostatics.

Bulletin of experimental biology and medicine·2025

Same author

Effect of Soybean Trypsin Inhibitor on Biochemical Parameters of the Blood under Conditions of Water and Food Deprivation in Rats.

Bulletin of experimental biology and medicine·2025

Same author

Consequences of Reprogrammed CD8<sup>+</sup> T-Cell Therapy for Lewis Lung Carcinoma Cells and Neovasculogenesis in C57BL/6 Mice.

Bulletin of experimental biology and medicine·2025

Search research articles

Related Experiment Video

Updated: May 21, 2026

Isolation of High-density Lipoproteins for Non-coding Small RNA Quantification

Isolation of High-density Lipoproteins for Non-coding Small RNA Quantification

Published on: November 28, 2016

[A simple method for quantification of modified low-density lipoproteins].

B B Shoĭbonov, V Iu Baronets, L F Panchenko

Patologicheskaia Fiziologiia I Eksperimental'Naia Terapiia

|June 20, 2012

Summary

A new method quantifies modified low-density lipoproteins (mLDL) in serum using polyvinylpyrrolidone (PVP). This simple, two-step process detects mLDL

More Related Videos

Enrichment of Native Lipoprotein Particles with microRNA and Subsequent Determination of Their Absolute/Relative microRNA Content and Their Cellular Transfer Rate

Enrichment of Native Lipoprotein Particles with microRNA and Subsequent Determination of Their Absolute/Relative microRNA Content and Their Cellular Transfer Rate

Published on: May 9, 2019

Isolation and Analysis of Plasma Lipoproteins by Ultracentrifugation

Isolation and Analysis of Plasma Lipoproteins by Ultracentrifugation

Published on: January 28, 2021

Related Experiment Videos

Last Updated: May 21, 2026

Isolation of High-density Lipoproteins for Non-coding Small RNA Quantification

Isolation of High-density Lipoproteins for Non-coding Small RNA Quantification

Published on: November 28, 2016

Enrichment of Native Lipoprotein Particles with microRNA and Subsequent Determination of Their Absolute/Relative microRNA Content and Their Cellular Transfer Rate

Enrichment of Native Lipoprotein Particles with microRNA and Subsequent Determination of Their Absolute/Relative microRNA Content and Their Cellular Transfer Rate

Published on: May 9, 2019

Isolation and Analysis of Plasma Lipoproteins by Ultracentrifugation

Isolation and Analysis of Plasma Lipoproteins by Ultracentrifugation

Published on: January 28, 2021

Area of Science:

Biochemistry
Clinical Chemistry
Immunology

Background:

Modified low-density lipoproteins (mLDL) are implicated in atherosclerosis.
Accurate quantification of mLDL is crucial for assessing cardiovascular risk.
Existing methods for mLDL detection can be complex and require sample fractionation.

Purpose of the Study:

To develop a simple and direct method for quantifying mLDL in human serum.
To validate the atherogenic potential of aggregated mLDL.

Main Methods:

Incubation of serum with 8.9% polyvinylpyrrolidone (PVP) solution.
Measurement of serum turbidity to quantify mLDL aggregation.
Experimental confirmation of atherogenicity using complement activation and platelet hyperaggregation assays.

Main Results:

A 10-minute incubation with 8.9% PVP induced complete mLDL aggregation in serum.
The method quantifies mLDL directly in serum without prior fractionation.
Aggregated mLDLs demonstrated pro-inflammatory and thrombogenic effects in independent assays.

Conclusions:

A straightforward, two-step method for mLDL quantification in serum has been established.
The method leverages PVP-induced mLDL aggregation and turbidity measurement.
The findings confirm the atherogenic nature of aggregated mLDL, highlighting its clinical relevance.