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Updated: May 21, 2026

A Modular Microfluidic Technology for Systematic Studies of Colloidal Semiconductor Nanocrystals

A Modular Microfluidic Technology for Systematic Studies of Colloidal Semiconductor Nanocrystals

Published on: May 10, 2018

Slip flow in colloidal crystals for ultraefficient chromatography.

Bingchuan Wei¹, Benjamin J Rogers, Mary J Wirth

¹Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, USA.

Journal of the American Chemical Society

|June 20, 2012

Summary

Enhanced fluid flow in colloidal crystals using slip flow significantly improves protein separation. This novel approach offers faster and more precise chromatography for biomolecules like antibodies.

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Published on: June 12, 2016

Area of Science:

Colloidal science
Chromatography
Biophysics

Background:

Traditional chromatography faces limitations in separation efficiency and speed.
Protein separations are crucial for biopharmaceutical development and analysis.

Purpose of the Study:

To investigate the application of slip flow in colloidal crystals for enhanced protein separation.
To evaluate the efficiency and speed of this method compared to conventional techniques.

Main Methods:

Utilizing colloidal crystals composed of 470 nm silica spheres with hydrocarbon modification.
Employing pressure-driven flow to induce slip flow phenomena.
Separating bovine serum albumin and a monoclonal antibody from its aggregates.

Main Results:

Slip flow in modified colloidal crystals resulted in enhanced volume flow rates and narrower fluid velocity distributions.
Bovine serum albumin separation achieved a zone 15-fold narrower than the Hagen-Poiseuille flow limit.
Monoclonal antibody separation from aggregates was accomplished in 40 seconds, a 10-fold increase in speed.

Conclusions:

Slip flow in colloidal crystals presents a significant advancement for protein chromatography.
This method offers unprecedented resolution and speed for separating biomolecules.
The findings have profound implications for improving protein analysis and purification techniques.