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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: May 21, 2026

ExCYT: A Graphical User Interface for Streamlining Analysis of High-Dimensional Cytometry Data

ExCYT: A Graphical User Interface for Streamlining Analysis of High-Dimensional Cytometry Data

Published on: January 16, 2019

Computational analysis of high-throughput flow cytometry data.

J Paul Robinson¹, Bartek Rajwa, Valery Patsekin

¹Purdue University Cytometry Laboratories, Purdue University, West Lafayette, IN 47907, USA. jpr@flowcyt.cyto.purdue.edu

Expert Opinion on Drug Discovery

|June 20, 2012

Summary

This summary is machine-generated.

High-throughput flow cytometry (HT-FC) is now a competitive technology for single-cell analysis and drug screening. Advances in hardware and analytics enable new applications in systems biology.

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Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction

Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction

Published on: August 15, 2013

Related Experiment Videos

Last Updated: May 21, 2026

ExCYT: A Graphical User Interface for Streamlining Analysis of High-Dimensional Cytometry Data

ExCYT: A Graphical User Interface for Streamlining Analysis of High-Dimensional Cytometry Data

Published on: January 16, 2019

Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction

Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction

Published on: August 15, 2013

Area of Science:

Biotechnology
Cellular Biology
Analytical Chemistry

Background:

Flow cytometry, a 40-year-old technology, is transitioning to high-throughput (HT) applications.
Historically focused on single-cell analysis, flow cytometry now offers competitive performance with imaging tools.

Purpose of the Study:

To discuss the evolution of flow cytometry towards high-throughput and high-content analysis.
To present arguments for adopting HT flow cytometry in pharmaceutical research and drug screening.

Main Methods:

Review of historical flow cytometry operations and recent technological advancements.
Discussion of hardware and analytical tool development for HT flow cytometry.

Main Results:

HT flow cytometry demonstrates strong capabilities for drug screening and functional studies in suspension cells.
Technological advancements have overcome previous limitations in sample throughput.

Conclusions:

HT flow cytometry is a powerful phenotypic analytical tool poised for adoption by the pharmaceutical community.
The technology's enhanced capacity enables exploration of systems biology questions previously unaddressed.