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Updated: May 21, 2026

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
Published on: February 10, 2023
Mitochondrial genome of Hemibagrus macropterus (Teleostei, Siluriformes)
Qing Zeng1, Hua Ye, Zuogang Peng
1The Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, School of Life Science, Southwest University, Beibei, Chongqing 400715, P.R. China.
Abstract:
The complete mitochondrial genome sequence of the Chinese catfish Hemibagrus macropterus was determined using long polymerase chain reaction (PCR). The complete mtDNA sequence was 16,530 bp in length and contained 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, and a control region (D-loop), the gene composition and order of which were identical to most of the other vertebrates. Two conserved sequence blocks (CSB-2 and CSB-3) in the control region can be easily identified in all available bagrid mitogenomes. The overall base composition of the heavy strand was 31.6% A, 27.7% T, 25.8% C, and 14.8% G, with an AT content of 59.3%. The mitogenome sequence of H. macropterus could be contributed to elucidate the evolutionary relationships among the bagrid catfishes, the systematics and evolution of which group remain poorly explored to date.
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