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Updated: May 21, 2026

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput
Published on: March 30, 2018
Expression profiling of ABA pathway transcripts indicates crosstalk between abiotic and biotic stress responses in
1Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China. zhulongch@wbgcas.cn
Abstract:
Recent breakthrough on identification and characterization of PYR/PYLs as ABA receptors enables us to better understand the perception, signaling and transportation of ABA in plant. Based on publicly available microarray data, transcriptional levels of ABA signaling pathway core components were compared after stress and phytohormone treatments, including these involved in ABA metabolism, signal transduction, and catabolism. The results showed that both abiotic and biotic stress treatments increased the expression levels of ABA key metabolism and catabolism transcripts. The expression levels of PYR/PYLs were down-regulated and these of PP2Cs and ABFs were uniformly up-regulated after exogenous ABA application and under stress conditions. The results indicated that the increased ratio of PP2Cs:PYR/PYLs might be required for activation of the downstream ABA signal pathway under both abiotic and biotic stress conditions. We concluded that abiotic and biotic stress responses shared ABA signal pathway in Arabidopsis.
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Transcription
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...

