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Updated: May 21, 2026

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip
13:34

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

Published on: September 29, 2012

Overexpression of ribosome binding protein 1 (RRBP1) in breast cancer

Deepthi Telikicherla1, Arivusudar Marimuthu, Manoj Kumar Kashyap

  • 1Institute of Bioinformatics, International Tech Park, Bangalore 560 066, India. jagadeesha@ibioinformatics.org.

Clinical Proteomics
|June 20, 2012
PubMed

Insights

Ribosome binding protein 1 (RRBP1) is upregulated in breast cancer at the mRNA level. This study found RRBP1 protein overexpression in 84% of breast carcinomas, suggesting its potential as a breast cancer biomarker.

Area of Science:

  • Molecular biology
  • Cancer research
  • Proteomics

Background:

  • Breast carcinoma involves molecular alterations at genomic, transcriptomic, and proteomic levels.
  • Identifying genes upregulated at mRNA level requires protein-level validation for biomarker potential.

Purpose of the Study:

  • To identify and validate candidate genes upregulated at the mRNA level in breast cancer.
  • To investigate the protein expression of ribosome binding protein 1 (RRBP1) in breast carcinoma.

Main Methods:

  • Utilized public gene expression databases to identify candidate genes.
  • Conducted literature search to pinpoint RRBP1 for further investigation.
  • Performed immunohistochemical labeling on breast cancer tissue microarrays to assess RRBP1 protein expression.

Main Results:

  • Ribosome binding protein 1 (RRBP1) mRNA is upregulated in breast cancer across multiple studies.
  • RRBP1 protein was found to be overexpressed in 84% of the tested breast carcinoma cases.
  • Subcellular localization of RRBP1 was predominantly cytoplasmic, with strong perinuclear staining.

Conclusions:

  • RRBP1 is significantly overexpressed in breast carcinoma at the protein level.
  • RRBP1 warrants further investigation as a potential diagnostic biomarker for breast cancer.
  • This study highlights the utility of integrating public data with experimental validation in clinical proteomics.

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