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Published on: March 18, 2019

D-chiro-inositol negatively regulates the formation of multinucleated osteoclasts by down-regulating NFATc1

Jungeun Yu1, Seunga Choi, Eui-Soon Park

  • 1Department of Microbiology and Molecular Biology, Chungnam National University, 220 Gung-dong, Yuseong-gu, Daejeon, 305-764, Republic of Korea.

Journal of Clinical Immunology
|June 20, 2012
PubMed
Abstract

Insights

D-chiro-inositol inhibits osteoclast formation by reducing cell fusion and down-regulating NFATc1 expression. This suggests D-chiro-inositol may treat inflammatory bone loss and osteoporosis.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Bone Metabolism

Background:

  • Osteoclasts (OCs) are critical for bone resorption; their accelerated activity contributes to diseases like osteoporosis.
  • D-pinitol is converted to D-chiro-inositol, a potential insulin-like mediator.
  • Understanding D-chiro-inositol's role in OC differentiation is crucial for bone health.

Purpose of the Study:

  • To investigate the effect of D-chiro-inositol on osteoclast differentiation.
  • To determine if D-chiro-inositol influences osteoclast formation and gene expression.

Main Methods:

  • Osteoclastogenesis cultures were used with tartrate-resistant acid phosphatase (TRAP) staining.
  • Cell fusion assays were performed with purified OC precursors.
  • The impact of D-chiro-inositol on nuclear factor of activated T cells c1 (NFATc1) and OC marker gene expression was analyzed.

Main Results:

  • D-chiro-inositol inhibited receptor activator of NF-κB ligand-induced OC differentiation.
  • Multinucleated OC formation via cell-cell fusion was dose-dependently reduced by D-chiro-inositol.
  • D-chiro-inositol suppressed osteoclastogenic gene expression by down-regulating NFATc1.

Conclusions:

  • D-chiro-inositol negatively regulates osteoclastogenesis by inhibiting multinucleated OC formation.
  • Down-regulation of NFATc1 by D-chiro-inositol leads to reduced OC marker gene expression.
  • D-chiro-inositol shows potential as a therapeutic agent for inflammatory bone diseases and diabetic osteoporosis.

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