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Updated: Aug 23, 2026

Protein Membrane Overlay Assay: A Protocol to Test Interaction Between Soluble and Insoluble Proteins in vitro
Published on: August 14, 2011
Immobilon-P transfer membrane: applications and utility in protein biochemical analysis
1Millipore Corporation, Bedford, MA 01730.
Abstract:
Polyvinylidene fluoride membrane for protein-blotting applications was introduced by Millipore Corporation in 1986. The membrane, designated variously as Immobilon-PVDF, Immobilon-P Transfer Membrane and Immobilon-P, has been widely used in protein biochemical analysis. Applications developed using Millipore's polyvinylidene fluoride membrane span immunochemistry, cell biology and protein structural characterization. Additionally, protein analysis on Immobilon-P membrane has been interfaced to a range of separation methodologies including high-pressure liquid chromatography and both one- and two-dimensional electrophoresis.
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