Related Experiment Video
Updated: May 21, 2026

Metagenomic Analysis of Silage
Published on: January 13, 2017
[Analysis of microbial diversity in rectum of calf in large-scale cattle farm]
Qiang Xu1, Lichao Kang, Xinwen Bo
1The Breed and Biotechnology Key Laboratory of Sheep in Reproduction and Construction Group of Xinjiang, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi 832000, China. cszero6130@163.com
Objective:
To analyze the diversity of bacterial community in rectum of diarrheic calves, and differences with health calves.
Methods:
16S rRNA clone libraries were constructed, positive clones were digested by Msp I and Hha I for restriction fragment length polymorphism (RFLP), and then a phylogenetic tree was depicted based on the 16S rRNA sequencing, to confirm the compose of microbe in the diarrheic calf rectum.
Results:
The positive rate of clone was 98.75% (474/480) in diarrheic calves, the dominant bacteria included Lactobacillus (14%), Enterococcus (10%) and Escherichia (8%). The positive rate of clone was 96.45% (488/506) in health samples, the dominant bacteria included Clostridium (13%), Bifidobacterium (8%), Megasphaera (5%).
Conclusion:
Complexity and diversity of bacterial community in rectum in 2 weeks old calves had their own features, and significant increase of Lactobacillus, Enterococcus and Escherichia was found in diarrhea calves.
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