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DEAD-box RNA helicases as transcription cofactors

Frances V Fuller-Pace1, Samantha M Nicol

  • 1Division of Cancer Research, Medical Research Institute, University of Dundee, Ninewells Hospital and Medical School, Dundee, United Kingdom.

Methods in Enzymology
|June 21, 2012
PubMed

Insights

DEAD box RNA helicases DDX5 (p68) and DDX17 (p72) regulate transcription by interacting with transcription factors. Protocols investigate their interactions and SUMOylation in transcriptional regulation.

Area of Science:

  • Molecular Biology
  • Gene Regulation

Background:

  • DEAD box RNA helicases, including DDX5 (p68) and DDX17 (p72), have diverse cellular functions.
  • These helicases are implicated in RNA processing, alternative splicing, and transcriptional regulation.
  • They act as coactivators or cosuppressors by interacting with transcription factors.

Purpose of the Study:

  • To describe protocols for investigating the factors influencing p68 and p72 function in transcriptional regulation.
  • To explore the interactions of p68 and p72 with transcription factors and machinery.
  • To examine the role of SUMOylation in p68 and p72 activity.

Main Methods:

  • Investigating protein-protein interactions between DDX5/DDX17 and transcription factors.
  • Analyzing interactions with components of the transcription machinery.
  • Studying posttranslational modification by SUMO (Small Ubiquitin-related Modifier).

Main Results:

  • DDX5 and DDX17 interact with transcription factors and regulate their activity.
  • These helicases are integral to the transcription machinery.
  • SUMOylation influences the function of DDX5 and DDX17 in transcription.

Conclusions:

  • DDX5 and DDX17 are key regulators of transcription through protein interactions and modifications.
  • Understanding these interactions and SUMOylation is crucial for deciphering their roles in gene expression.
  • The described protocols provide a framework for further research into DEAD box helicase functions.

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