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Updated: May 21, 2026
14:04
Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy
Published on: April 25, 2021
A highly sensitive "switch-on" fluorescent probe for protein quantification and visualization based on
Fangfang Wang1, Jiying Wen, Lingyun Huang
1College of Chemistry, Beijing Normal University, Beijing, 100875, PR China.
Chemical Communications (Cambridge, England)
|June 21, 2012
Summary
A novel fluorescent probe enables sensitive protein detection and imaging. This "switch-on" technology offers a fast, affordable method for analyzing serum proteins and disease biomarkers.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Molecular Imaging
Background:
- Protein quantification is crucial for disease diagnosis.
- Existing methods can be complex or lack sensitivity.
- Need for efficient and sensitive protein detection tools.
Purpose of the Study:
- To develop a highly sensitive and water-soluble fluorescent probe.
- To utilize aggregation-induced emission (AIE) characteristics for enhanced detection.
- To enable rapid quantification and visualization of proteins.
Main Methods:
- Synthesis of a novel "switch-on" fluorescent probe.
- Characterization of probe properties, including water solubility and AIE.
- Application of the probe for assaying complete serum proteins.
- Validation for detecting disease-marker proteins.
Main Results:
- The developed probe exhibits high sensitivity and water solubility.
- Aggregation-induced emission characteristics were confirmed.
- Successful quantification and visualization of complete serum proteins were achieved.
- Effective detection of disease-marker proteins demonstrated.
Conclusions:
- The AIE-based fluorescent probe provides a sensitive platform for protein analysis.
- This method offers a rapid, economical, and effective approach for clinical diagnostics.
- Potential for widespread application in protein quantification and disease biomarker detection.
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