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Three-dimensional Optical-resolution Photoacoustic Microscopy
Published on: May 3, 2011
Correcting photoacoustic signals for fluence variations using acousto-optic modulation
K Daoudi1, A Hussain, E Hondebrink
1Biomedical Photonic Imaging group, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, PO Box 217, 7500 AE Enschede, The Netherlands.
Optics Express
|June 21, 2012
Summary
This study introduces a new method for quantitative photoacoustic mapping by tagging light deep within tissues. The technique accurately corrects for light variations, improving chromophore concentration measurements.
Area of Science:
- Biomedical Optics
- Photoacoustics
- Medical Imaging
Background:
- Quantitative photoacoustic imaging aims to map chromophore concentrations.
- Variations in light fluence deep within tissues pose a significant challenge.
- Accurate measurements require correction for these fluence variations.
Purpose of the Study:
- To present a theoretical concept for quantitative photoacoustic mapping.
- To develop a method for correcting fluence variations in photoacoustic measurements.
- To validate the proposed technique experimentally.
Main Methods:
- Derivation of a formula relating local absorption coefficient to noninvasively measured quantities.
- Monte Carlo simulations for theoretical validation.
- Experimental validation using acousto-optic modulation with focused ultrasound to tag photons.
Main Results:
- The derived formula allows for quantitative mapping of chromophore concentrations.
- Experimental results demonstrate correction of photoacoustic signal variations caused by fluence changes exceeding one order of magnitude.
- Correction accuracy of 5% was achieved for absorbing insertions at various depths.
Conclusions:
- The proposed technique provides a viable strategy for quantitative photoacoustic mapping.
- The method effectively corrects for fluence variations, enhancing measurement accuracy.
- This approach has potential for improved deep-tissue optical imaging and diagnostics.
