Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: May 21, 2026

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

Nanowire transistor-based ultrasensitive virus detection with reversible surface functionalization.

Pei-Ling Chiang¹, Tzu-Chi Chou, Tzu-Heng Wu

¹Department of Chemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 10617 Taiwan, R.O.C.

Chemistry, an Asian Journal

|June 21, 2012

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Synergistic Integration of Redox-Active Deep Eutectic Gel Electrolyte and Hierarchical CNT@ZIF-Derived Porous Carbon for Advanced Micro-Supercapacitors.

Small (Weinheim an der Bergstrasse, Germany)·2026

Same author

Electrolyte-Induced Interfacial ZnMn<sub>2</sub>O<sub>4</sub> Formation on MnO<sub>2</sub>@MOF-5 Cathodes for Ultra-Stable Aqueous Zinc-Ion Batteries.

Small (Weinheim an der Bergstrasse, Germany)·2026

Same author

Broadband high-performance terahertz polarizers by nanoimprint lithography for advanced applications.

Optics express·2025

Same author

In Situ Oxidative Polymerization of PEDOT, Reducing the TBP Dosage, and Synergizing with Spiro-OMeTAD to Enhance the Efficiency and Stability of Perovskite Solar Cells.

ACS applied materials & interfaces·2025

Same author

Glycerol Electro-Oxidation to Dihydroxyacetone with Coupled Hydrogen Production via In Situ Optimization of Water Oxidation Intermediates.

Small (Weinheim an der Bergstrasse, Germany)·2025

Same author

Ultrasensitive Quantification of Thyroid-Stimulating Hormone and Thyroxine by Nanoelectronic SnS<sub>2</sub> Transistor Sensors.

ACS sensors·2025

This study presents a reusable silicon nanowire field-effect transistor (SiNW-FET) biosensor for highly sensitive detection of H5N2 avian influenza virus (AIV). The novel reversible surface functionalization enables ultrasensitive detection of AIV in dilute solutions.

Area of Science:

Nanotechnology
Biosensing
Virology

Background:

Avian influenza virus (AIV) poses a significant threat to global health and poultry industries.
Current detection methods for AIV can be time-consuming and lack sensitivity for early-stage detection.
Development of rapid, ultrasensitive, and reusable biosensors is crucial for effective AIV surveillance and control.

Purpose of the Study:

To develop and validate a reusable silicon nanowire field-effect transistor (SiNW-FET) biosensor for ultrasensitive detection of H5N2 avian influenza virus (AIV).
To establish a novel reversible surface functionalization strategy for the SiNW-FET biosensor using disulfide linkers.
To demonstrate the capability of the functionalized SiNW-FET biosensor to detect extremely low concentrations of H5N2 AIV.

Main Methods:

More Related Videos

Preparation of Silicon Nanowire Field-effect Transistor for Chemical and Biosensing Applications

Preparation of Silicon Nanowire Field-effect Transistor for Chemical and Biosensing Applications

Published on: April 21, 2016

Related Experiment Videos

Last Updated: May 21, 2026

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

Preparation of Silicon Nanowire Field-effect Transistor for Chemical and Biosensing Applications

Preparation of Silicon Nanowire Field-effect Transistor for Chemical and Biosensing Applications

Published on: April 21, 2016

Surface modification of SiNW-FET with 3-mercaptopropyltrimethoxysilane (MPTMS) to create MPTMS/SiNW-FET.
Reversible immobilization of receptor molecules (monoclonal antibody against H5N2) via disulfide bond formation.
Verification of surface functionalization using fluorescence and electrical measurements.
Atomic force microscopy (AFM) to analyze the surface topography after H5N2 AIV detection.

Main Results:

Successful reversible surface functionalization of SiNW-FET was achieved and verified.
The MPTMS/SiNW-FET biosensor demonstrated ultrasensitive detection of H5N2 AIV at concentrations as low as approximately 10(-17) M.
AFM imaging confirmed the presence of detected H5N2 AIV particles on the functionalized SiNW-FET surface.

Conclusions:

The developed SiNW-FET biosensor offers a promising platform for ultrasensitive and reusable detection of H5N2 AIV.
The reversible surface functionalization strategy is effective for immobilizing biomolecules and enhances biosensor performance.
This technology has the potential for early and rapid diagnosis of avian influenza, aiding in disease control efforts.