Related Experiment Video
Updated: May 21, 2026

12:42
Imaging the Neutrophil Phagosome and Cytoplasm Using a Ratiometric pH Indicator
Published on: April 5, 2017
Imaging histamine in live basophils and macrophages with a fluorescent mesoionic acid fluoride
Nicola Kielland1, Marc Vendrell, Rodolfo Lavilla
1Institute for Biomedical Research, Barcelona Science Park, Spain.
Abstract:
Histamine is a biogenic amine with fundamental roles in circulatory and immune systems. We report a fluorescent small molecule (Histamine Blue) for imaging intracellular histamine in live basophils and macrophages. Histamine Blue is a fluorescent mesoionic acid fluoride that turns on upon reaction with histamine. The selective response of Histamine Blue enabled the visualization of intracellular histamine under different physiological conditions.

