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Reliable Method for Assessing Seed Germination, Dormancy, and Mortality under Field Conditions
Published on: November 6, 2016
[Study on seeds dormancy release and physiological change of Thesium chinense]
1Institute of Chinese Medicinal Materials, Nanjing Agricultural University, Nanjing 210095, China.
Objective:
To study the seeds dormancy release and physiological change of Thesium chinense.
Method:
To release dormancy, the seeds of T. chinense were treated with chemical reagent and stratification under 3-5 degrees C.
Result:
When washed with flowing water for 24 h, then soaked in 500 mg x L(-1) GA3 for 24 h, finally, treated with stratification method under 3-5 degrees C for 150 day, the split rate of T. chinense seeds reached 22%, crude fat decreased 50%, total sugar and dissolvability sugar increased 3-4 times, ABA decreased more than 90%, GA3 increased more than 5 times.
Conclusion:
The seeds dormancy of T. chinense can be released with the method of washing with flowing water for 24 h, then soaking in 500 mg x L(-1) GA3 for 24 h, finally, treated with stratification method under 3-5 degrees C for 150-180 day.
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