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Fluorescence in situ Hybridizations (FISH) for the Localization of Viruses and Endosymbiotic Bacteria in Plant and Insect Tissues
Published on: February 24, 2014
Tomato dwarf leaf virus, a New World begomovirus infecting tomato in Argentina
C G Vaghi Medina1, P M López Lambertini
1Instituto de Patología Vegetal (IPAVE) CIAP-INTA, Córdoba, Argentina.
Abstract:
Begomovirus infection is becoming a threat in fresh-market tomato in Argentina, where mixed infections with begomoviruses are common. The complete sequence of a begomovirus isolate infecting tomato sampled in Salta was molecularly characterized. Phylogenetic analysis showed that this virus isolate is closely related to previously reported Brazilian, Bolivian and Argentinean begomoviruses. The associated symptoms in Nicotiana benthamiana and Solanum lycopersicum were determined by biolistic delivery of infectious DNA-A and DNA-B clones. This begomovirus isolate induced leaf mottling, rugosity and dwarfing, and growth retardation in tomato. Based on these symptoms, we propose the name of tomato dwarf leaf virus (ToDLV) for this new begomovirus.
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