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Related Experiment Video

Updated: May 21, 2026

Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro

Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro

Published on: March 17, 2023

Apolipoprotein A5 internalized by human adipocytes modulates cellular triglyceride content.

Xiao-Yan Zheng¹, Shui-Ping Zhao, Bi-Lian Yu

¹Department of Cardiology, The Second Xiangya Hospital, Central South University, Changsha 410011, Hunan, P.R. China.

Biological Chemistry

|June 22, 2012

Summary

Apolipoprotein A5 (apoA5) is internalized by human fat cells, where it surrounds lipid droplets. This protein reduces cellular triglyceride storage, suggesting a new role in fat metabolism.

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Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis

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Published on: June 3, 2016

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Last Updated: May 21, 2026

Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro

Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro

Published on: March 17, 2023

Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis

Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis

Published on: June 3, 2016

Area of Science:

Biochemistry
Cell Biology
Metabolic Research

Background:

Apolipoprotein A5 (apoA5) influences plasma triglyceride (TG) levels.
Previous studies indicated apoA5 modulates TG metabolism in liver cells (hepatocytes).

Purpose of the Study:

To investigate apoA5 internalization by adipocytes (fat cells).
To determine if apoA5 regulates cellular triglyceride (TG) storage in adipocytes.

Main Methods:

Human preadipocytes were differentiated into mature adipocytes.
Pulse-chase experiments tracked apoA5 internalization and degradation.
Heparin and receptor-associated protein inhibited apoA5 uptake.
Confocal microscopy visualized apoA5 localization with lipid droplets and perilipin.
Western blot analysis confirmed apoA5 uptake.

Main Results:

ApoA5 was internalized by human adipocytes, with most remaining intracellular over 24 hours.
Uptake was significantly reduced by heparin and receptor-associated protein, indicating LDL receptor family involvement.
Internalized apoA5 localized around lipid droplets and co-localized with perilipin.
ApoA5 treatment significantly decreased cellular TG storage.

Conclusions:

ApoA5 is internalized by human adipocytes.
ApoA5 may function as a novel regulator of triglyceride storage in human adipocytes.