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Milk progesterone determination in buffaloes post-insemination
1Division of Cattle Physiology, National Dairy Research Institute, Karnal, India.
Summary
Milk progesterone testing can diagnose pregnancy in buffaloes. This method accurately identifies non-pregnant animals and can detect silent heats or clinical disorders.
Area of Science:
- Reproductive biology
- Veterinary medicine
- Dairy science
Background:
- Accurate pregnancy diagnosis is crucial for efficient dairy herd management.
- Milk progesterone levels fluctuate with the oestrous cycle and pregnancy status.
Purpose of the Study:
- To evaluate the efficacy of milk progesterone levels for pregnancy diagnosis in buffaloes.
- To determine the accuracy of milk progesterone testing at different days post-insemination.
Main Methods:
- Milk samples were collected from 17 buffaloes from insemination up to 30 days.
- Progesterone levels were measured to track changes in pregnant and non-pregnant animals.
- A cut-off progesterone level was established for pregnancy diagnosis.
Main Results:
- Pregnant buffaloes showed continuously increasing progesterone levels post-insemination.
- Non-pregnant buffaloes exhibited a decline in progesterone prior to oestrus.
- Pregnancy diagnosis accuracy was 60-75% by day 24, with 100% accuracy for non-pregnancy detection.
Conclusions:
- Milk progesterone assay is a viable tool for pregnancy diagnosis in buffaloes, particularly for identifying non-pregnant animals.
- Variable oestrous cycle length can influence diagnostic accuracy.
- The assay may also aid in identifying silent heats and clinical disorders.