Related Experiment Video

Updated: May 21, 2026

Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase
11:01

Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase

Published on: November 23, 2016

(E)-4-Amino-N'-(2-nitro-benzyl-idene)benzohydrazide

Zhong-Feng Shi, Jia-Ming Li

    Acta Crystallographica. Section E, Structure Reports Online
    |June 22, 2012
    PubMed

    More Related Videos

    Synthesis of Antiviral Tetrahydrocarbazole Derivatives by Photochemical and Acid-catalyzed C-H Functionalization via Intermediate Peroxides (CHIPS)06:34

    Synthesis of Antiviral Tetrahydrocarbazole Derivatives by Photochemical and Acid-catalyzed C-H Functionalization via Intermediate Peroxides (CHIPS)

    Published on: June 20, 2014

    Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly09:34

    Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

    Published on: February 6, 2020

    Related Concept Videos

    Diazonium Group Substitution: –OH and –H01:19

    Diazonium Group Substitution: –OH and –H

    Nitrous acid, a weak acid, is prepared in situ via the reaction of sodium nitrite with a strong acid under cold conditions. This nitrous acid prepared in situ reacts with primary arylamines to form arenediazonium salts. Such reactions are known as diazotization reactions. As shown in Figure 1, the formation of arenediazonium salts begins with the decomposition of nitrous acid in an acidic solution to give nitrosonium ions.
    Electrophilic Aromatic Substitution: Nitration of Benzene01:20

    Electrophilic Aromatic Substitution: Nitration of Benzene

    The nitration of benzene is an example of an electrophilic aromatic substitution reaction. It involves the formation of a very powerful electrophile, the nitronium ion, which is linear in shape. The reaction occurs through the interaction of two strong acids, sulfuric and nitric acid.
    1° Amines to Diazonium or Aryldiazonium Salts: Diazotization with NaNO2 Mechanism01:37

    1° Amines to Diazonium or Aryldiazonium Salts: Diazotization with NaNO2 Mechanism

    Nitrous acid is a relatively weak and unstable acid prepared in situ by the reaction of sodium nitrite and cold, dilute hydrochloric acid. In an acidic solution, the nitrous acid undergoes protonation when it loses water to form a nitrosonium ion—an electrophile. Nitrous acid reacts with primary amines to give diazonium salts. The reaction is called diazotization of primary amines.
    Diazonium Group Substitution with Halogens and Cyanide: Sandmeyer and Schiemann Reactions01:20

    Diazonium Group Substitution with Halogens and Cyanide: Sandmeyer and Schiemann Reactions

    Arenediazonium substitution reactions occur when the diazonium group is substituted by various functional groups such as halides, hydroxyl, nitrile, etc. For instance, arenediazonium salts react with copper(I) salts of chloride, bromide, or cyanide to form corresponding aryl chlorides, bromides, and nitriles. These reactions are named Sandmeyer reactions. Although the mechanism of this reaction is complicated, as illustrated in Figure 1, they are believed to progress via an aryl copper...
    Nucleophilic Aromatic Substitution of Aryldiazonium Salts: Aromatic SN101:14

    Nucleophilic Aromatic Substitution of Aryldiazonium Salts: Aromatic SN1

    Treating arylamines with nitrous acid gives aryldiazonium salts that are effective substrates in nucleophilic aromatic substitution reactions. The diazonio group in these salts can be easily displaced by different nucleophiles, yielding a wide variety of substituted benzenes. The leaving group departs as nitrogen gas, and this easy elimination is the driving force for the substitution reaction.
    In the Sandmeyer reaction, for example, the diazonio group is replaced by a chloro, bromo, or cyano...
    Nucleophilic Aromatic Substitution: Elimination–Addition01:11

    Nucleophilic Aromatic Substitution: Elimination–Addition

    Simple aryl halides do not react with nucleophiles. However, nucleophilic aromatic substitutions can be forced under certain conditions, such as high temperatures or strong bases. The mechanism of substitution under such conditions involves the highly unstable and reactive benzyne intermediate. Benzyne contains equivalent carbon centers at both ends of the triple bond, each of which is equally susceptible to nucleophilic attack. This 50–50 distribution of products is confirmed through isotopic...