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Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
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Low serum glutathione peroxidase activity is associated with increased cardiovascular mortality in individuals with

Brian Buijsse1, Duk-Hee Lee, Lyn Steffen

  • 1Division of Epidemiology and Community Health, School of Public Health, University of Minnesota, Minneapolis, Minnesota, United States of America.

Plos One
|June 22, 2012
PubMed

Insights

High glutathione peroxidase 3 (GPx3) activity may reduce cardiovascular disease (CVD) mortality. Low GPx3 and low HDL cholesterol significantly increase the risk of death from CVD.

Area of Science:

  • Biochemistry
  • Cardiovascular Science
  • Epidemiology

Background:

  • Oxidized low-density lipoprotein (LDL) is a key initiator of atherosclerosis.
  • Serum glutathione peroxidase 3 (GPx3) plays a role in reducing oxidized lipids.
  • The relationship between GPx3 activity and cardiovascular disease (CVD) mortality requires further investigation.

Purpose of the Study:

  • To investigate the association between serum GPx3 activity and CVD mortality.
  • To determine if higher GPx3 activity is protective against CVD death.
  • To explore potential interactions between GPx3 activity and other risk factors like HDL cholesterol.

Main Methods:

  • A nested case-control study was conducted using samples from the Minnesota Heart Survey.
  • 130 participants who died of CVD and 240 controls were analyzed for GPx3 activity.
  • Logistic regression models were used to calculate adjusted odds ratios (ORs) for CVD mortality across GPx3 quartiles.

Main Results:

  • An inverse correlation was observed between GPx3 activity and CVD mortality (P for trend = 0.02).
  • This protective association was significant in individuals with below-median HDL cholesterol levels (P for interaction = 0.006).
  • In participants with low HDL cholesterol, increasing GPx3 quartiles showed a significant trend of reduced CVD mortality (P for trend = 0.002).

Conclusions:

  • Individuals with both low HDL cholesterol and low GPx3 activity face a substantially elevated risk of death from CVD.
  • GPx3 activity may be a significant biomarker for cardiovascular risk, particularly in specific patient subgroups.
  • These findings highlight the complex interplay between lipid metabolism and antioxidant defense in cardiovascular health.
Abstract

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