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Updated: May 11, 2026

Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA
Published on: September 11, 2013
New insights into nucleosome and chromatin structure: an ordered state or a disordered affair?
Karolin Luger1, Mekonnen L Dechassa, David J Tremethick
1Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, Colorado 80523, USA. Karolin.Luger@ColoState.edu
Abstract:
The compaction of genomic DNA into chromatin has profound implications for the regulation of key processes such as transcription, replication and DNA repair. Nucleosomes, the repeating building blocks of chromatin, vary in the composition of their histone protein components. This is the result of the incorporation of variant histones and post-translational modifications of histone amino acid side chains. The resulting changes in nucleosome structure, stability and dynamics affect the compaction of nucleosomal arrays into higher-order structures. It is becoming clear that chromatin structures are not nearly as uniform and regular as previously assumed. This implies that chromatin structure must also be viewed in the context of specific biological functions.
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