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Updated: May 21, 2026

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
Published on: March 8, 2020
New alkyl-phosphate bonded stationary phases for liquid chromatographic separation of biologically active compounds
Szymon Bocian1, Alicja Nowaczyk, Boguslaw Buszewski
1Department of Environmental Chemistry and Bioanalysis, Faculty of Chemistry, Nicolaus Copernicus University, Torun, Poland.
Abstract:
A new type of bonded stationary phase for liquid chromatography, with the properties of immobilized artificial membranes, has been synthesized. Alkyl-phosphate adsorbents were obtained by modification of aminopropyl silica gel. The structures of the synthesized materials were confirmed by use of instrumental techniques--elemental analysis, infrared spectroscopy (FTIR), and (13)C and (29)Si CP/MAS NMR. Analysis revealed that the adsorbents mimic the phospholipids present in natural cell membranes. The new synthesized alkyl-phosphate stationary phases may be used for liquid chromatographic separation of biologically active compounds of different polarity.
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