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Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
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Selection of human Fab libraries by phage display

Christoph Rader1

  • 1Experimental Transplantation and Immunology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA. raderc@mail.nih.gov

Methods in Molecular Biology (Clifton, N.J.)
|June 23, 2012
PubMed

This protocol describes the selection of human antibody libraries in Fab format by phage display. It includes panning against immobilized antigens, biotinylated antigens in solution, and cell surface antigens.

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