Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

DVD-IgG1 antibody-drug conjugates: Expanding the landscape of targeted cancer therapy.

Current opinion in chemical biology·2026
Same author

Targeting mGlyR with nanobodies for depression.

Nature communications·2026
Same author

Engineered antibodies that stabilize drug-modified KRAS<sup>G12C</sup> neoantigens enable selective and potent cross-HLA immunotherapy.

Nature communications·2025
Same author

ROR2-specific CAR T cells are effective against hematologic and solid tumors and well tolerated in mice.

Cell reports. Medicine·2025
Same author

Second Generation Tiancimycin-Based Antibody-Drug Conjugates Enabled by Highly Efficient Semi-synthetic Approach Specifically Targeting B-Cell Malignancies.

JACS Au·2025
Same author

Potentiating CD20 monoclonal antibody therapy by targeting complement C3 fragments covalently deposited on lymphoma cells.

Blood·2025

Related Experiment Video

Updated: May 21, 2026

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
12:55

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

Published on: January 17, 2015

Selection of human Fab libraries by phage display.

Christoph Rader1

  • 1Experimental Transplantation and Immunology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA. raderc@mail.nih.gov

Methods in Molecular Biology (Clifton, N.J.)
|June 23, 2012
PubMed
Summary

This study details selecting human antibody libraries using phage display technology. Methods cover panning against various antigen formats for antibody discovery.

More Related Videos

Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity
12:31

Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity

Published on: May 1, 2018

Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing
12:04

Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing

Published on: October 3, 2018

Related Experiment Videos

Last Updated: May 21, 2026

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
12:55

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

Published on: January 17, 2015

Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity
12:31

Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity

Published on: May 1, 2018

Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing
12:04

Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing

Published on: October 3, 2018

Area of Science:

  • Biotechnology
  • Immunology
  • Molecular Biology

Background:

  • Phage display is a powerful technique for selecting specific antibodies from large libraries.
  • Antibody selection protocols need optimization for different antigen formats to maximize success.

Purpose of the Study:

  • To describe a comprehensive protocol for selecting human antibody fragments (Fab) using phage display.
  • To detail methods for panning antibody libraries against diverse antigen presentations.

Main Methods:

  • Selection of human antibody libraries in the Fab format.
  • Phage display panning against immobilized antigens.
  • Phage display panning against biotinylated antigens in solution.
  • Phage display panning against cell surface antigens.

Main Results:

  • Successful selection of human Fab antibodies against various antigen formats.
  • Demonstration of the versatility of phage display for antibody discovery.
  • Establishment of a robust protocol for antibody library screening.

Conclusions:

  • The described phage display protocol is effective for selecting human Fab antibodies.
  • The methods are adaptable for different antigen types, including cell surface targets.
  • This protocol provides a foundation for antibody discovery and engineering.