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Immunoprecipitation01:20

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...

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Related Experiment Video

Updated: May 21, 2026

Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation
07:57

Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation

Published on: August 21, 2019

Kinetic screening in the antibody development process.

Michael Schräml1, Matthias Biehl

  • 1Roche Diagnostics GmbH, Penzberg, Germany. michael.schraeml@roche.com

Methods in Molecular Biology (Clifton, N.J.)
|June 23, 2012
PubMed
Summary
This summary is machine-generated.

This study presents a new kinetic screening method using surface plasmon resonance biosensors to select custom antibodies. The protocol efficiently ranks antibodies based on kinetic signatures for scientific and pharmaceutical applications.

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Related Experiment Videos

Last Updated: May 21, 2026

Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation
07:57

Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation

Published on: August 21, 2019

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms
15:27

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms

Published on: April 17, 2017

Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations
09:07

Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations

Published on: August 22, 2019

Area of Science:

  • Biochemistry
  • Immunology
  • Biotechnology

Background:

  • Selecting custom antibodies is crucial for scientific, diagnostic, and pharmaceutical applications.
  • Existing methods may not comprehensively characterize antibody kinetics.

Purpose of the Study:

  • To describe a novel kinetic screening protocol for antibody selection.
  • To enable efficient ranking and selection of high-quality antibodies based on kinetic profiles.

Main Methods:

  • Utilized a Biacore A100 surface plasmon resonance (SPR) biosensor instrument.
  • Developed an assay employing an Fc-specific antibody capture system for oriented immobilization.
  • Implemented a single antigen injection method to determine multiple kinetic parameters.

Main Results:

  • The protocol determines six key screening parameters, including kinetic rate constants and valence mode.
  • Demonstrated efficient capture and orientation of antibodies from complex mixtures like hybridoma supernatants.
  • Enabled comprehensive characterization of antibody kinetic signatures.

Conclusions:

  • The described SPR-based kinetic screening method provides a robust approach for selecting superior antibodies.
  • This protocol facilitates the identification of antibodies with desired kinetic properties for specific applications.
  • The method offers a valuable tool for researchers in antibody discovery and development.