Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
DNA Bacteriophages01:26

DNA Bacteriophages

Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
Lytic Cycle of Bacteriophages01:30

Lytic Cycle of Bacteriophages

Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the lytic replication...
Methods of Classification and Identification01:28

Methods of Classification and Identification

Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...
Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Seaweed-derived fucoidans and rhamnan sulfates serve as potent anti-SARS-CoV-2 agents with potential for prophylaxis.

Carbohydrate polymers·2024
Same author

Protein coupled thionine acetate probed silica nanoparticles: An integrated laser-assisted therapeutic approach for treating cancer.

Bioorganic chemistry·2024
Same author

Cyclic AMP binding to a universal stress protein in Mycobacterium tuberculosis is essential for viability.

The Journal of biological chemistry·2024
Same author

Clinical and CBCT Assessment of Role of Bisphosphonate on Osteotomy Site and Implant Surface.

Journal of pharmacy & bioallied sciences·2024
Same author

Novel Compound Heterozygous Mutations of LIG4 Gene in an Indian <i>LIG4</i> Syndrome Patient with Severe Microcephaly: Case Report, <i>In-silico</i> Analysis and Systematic Review.

Current pediatric reviews·2024
Same author

Genetic mechanism regulating diversity in the placement of eyes on the head of animals.

Proceedings of the National Academy of Sciences of the United States of America·2024

Related Experiment Video

Updated: May 21, 2026

'Bioluminescent' Reporter Phage for the Detection of Category A Bacterial Pathogens
11:31

'Bioluminescent' Reporter Phage for the Detection of Category A Bacterial Pathogens

Published on: July 8, 2011

Bacteriophage based probes for pathogen detection.

Amit Singh1, Denis Arutyunov, Christine M Szymanski

  • 1Department of Electrical and Computer Engineering, University of Alberta, Edmonton, AB T6G 2V4, Canada. amit2@ualberta.ca

The Analyst
|June 23, 2012
PubMed
Summary
This summary is machine-generated.

Bacteriophages and their receptor binding proteins (RBPs) offer precise tools for detecting pathogenic bacteria. Immobilized phages or RBPs enable rapid, specific bacterial capture and detection, minimizing false positives.

More Related Videos

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
14:04

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Published on: May 8, 2013

In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA
11:15

In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA

Published on: May 21, 2015

Related Experiment Videos

Last Updated: May 21, 2026

'Bioluminescent' Reporter Phage for the Detection of Category A Bacterial Pathogens
11:31

'Bioluminescent' Reporter Phage for the Detection of Category A Bacterial Pathogens

Published on: July 8, 2011

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
14:04

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Published on: May 8, 2013

In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA
11:15

In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA

Published on: May 21, 2015

Area of Science:

  • Microbiology and Biotechnology
  • Biosensor Development
  • Pathogen Detection

Background:

  • Accurate and rapid detection of pathogenic bacteria is crucial for disease control in humans, animals, and plants.
  • Developing highly specific biological probes is essential to minimize false positives in bacterial detection.
  • Bacteriophages, viruses that infect bacteria, exhibit precise host recognition via receptor binding proteins (RBPs).

Purpose of the Study:

  • To review pathogen detection strategies utilizing immobilized bacteriophages and recombinant RBPs.
  • To highlight the advantages of RBP-based molecular probes for selective bacterial capture and detection.

Main Methods:

  • Review of literature on bacteriophage and RBP applications in pathogen detection.
  • Focus on methods involving immobilized phage virions and purified recombinant RBPs.
  • Discussion of techniques for selective bacterial capture on solid surfaces and subsequent detection.

Main Results:

  • Bacteriophages and their RBPs provide high specificity for bacterial recognition.
  • Immobilized phages and RBPs can be used to create probes for selective bacterial capture.
  • These probes facilitate quick and automated detection of bacterial binding events.

Conclusions:

  • Bacteriophage virions and RBPs are promising candidates for developing specific pathogen detection probes.
  • RBP-based molecular probes offer significant advantages for rapid and accurate bacterial identification.
  • This approach holds potential for improving disease diagnosis and public health surveillance.