Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Estrogen-binding proteins in the human postmenopausal uterus.

W E Gibbons, V C Buttram, P K Besch

    American Journal of Obstetrics and Gynecology
    |November 15, 1979
    PubMed
    Summary

    Human uterine cytosol contains two estrogen-binding proteins acting as receptors. The high-affinity receptor (10(-10)M) is key for estrogen response, while the low-affinity receptor

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    beta-Amyloid1-40 increases expression of beta-amyloid precursor protein in neuronal hybrid cells.

    Journal of neurochemistry·1995
    Same author

    Induction of c-fos mRNA in the arcuate nucleus of normal and mutant growth hormone-deficient mice by a synthetic non-peptidyl growth hormone secretagogue.

    Neuroreport·1995
    Same author

    Alpha-1 subunits of voltage gated Ca2+ channels in the mesencephalon x neuroblastoma hybrid cell line MES23.5.

    Neuroscience·1995
    Same author

    Cell death induced by beta-amyloid 1-40 in MES 23.5 hybrid clone: the role of nitric oxide and NMDA-gated channel activation leading to apoptosis.

    Brain research·1995
    Same author

    Does activation of the TAL1 gene occur in a majority of patients with T-cell acute lymphoblastic leukemia? A pediatric oncology group study.

    Blood·1995
    Same author

    5 alpha-Androstan-3 alpha,17 beta-diol is a hormone: stimulation of cAMP accumulation in human and dog prostate.

    The Journal of clinical endocrinology and metabolism·1995

    Area of Science:

    • Endocrinology
    • Molecular Biology
    • Gynecology

    Background:

    • Estrogen receptors mediate cellular responses to estrogen.
    • The human uterus possesses intracellular estrogen-binding proteins with receptor characteristics.
    • Understanding these receptors is crucial for reproductive health and hormone-related conditions.

    Purpose of the Study:

    • To characterize the high- and low-affinity estrogen-binding proteins in the human uterus.
    • To investigate the role of these proteins in estrogen stimulation and uterine function.
    • To determine the specificity and binding characteristics of these uterine estrogen receptors.

    Main Methods:

    • Scatchard analysis of uterine cytosol from postmenopausal and premenopausal women.
    • Partial purification of cytosol using ammonium sulfate fractionation.
    • Competition studies to assess receptor specificity for estradiol.

    Main Results:

    • Two distinct high-affinity (10(-10)M) and low-affinity (10(-9)M) estrogen-binding proteins were identified.
    • The high-affinity receptor was present in postmenopausal uterine cytosol without estrogen stimulation.
    • Both high- and low-affinity receptors were observed in estrogen-primed postmenopausal and premenopausal uterine systems.
    • Receptors demonstrated specificity for estradiol.

    Conclusions:

    • The human uterus possesses at least two distinct estrogen receptor components.
    • The high-affinity receptor likely plays a primary role in mediating estrogen's effects on the uterus.
    • The function of the low-affinity receptor requires further investigation.
    • These findings have implications for understanding estrogen's role in uterine physiology and pathology.

    Related Experiment Videos