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Technetium-99m-labeled ceftizoxime loaded long-circulating and pH-sensitive liposomes used to identify osteomyelitis
Soraya Maria Zandim Maciel Dias Ferreira1, Giselle Pires Domingos, Diego dos Santos Ferreira
1Faculdade de Farmácia, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, 31270-901, Belo Horizonte, Minas Gerais, Brazil.
Abstract:
Osteomyelitis is an infectious disease located in the bone or bone marrow. Long-circulating and pH-sensitive liposomes containing a technetium-99m-labeled antibiotic, ceftizoxime, (SpHL-(99m)Tc-CF) were developed to identify osteomyelitis foci. Biodistribution studies and scintigraphic images of bone infection or non infection-bearing rats that had been treated with these liposomes were performed. A high accumulation in infectious foci and high values in the target-non target ratio could be observed. These results indicate the potential of SpHL-(99m)Tc-CF as a potential agent for the diagnosis of bone infections.
Insights
New liposomes carrying a technetium-99m-labeled antibiotic show promise for diagnosing bone infections like osteomyelitis. These specialized liposomes accumulate in infected areas, aiding in accurate detection.
Area of Science:
- Nuclear medicine
- Biomedical engineering
- Infectious diseases
Background:
- Osteomyelitis is a challenging bone infection requiring accurate diagnostic tools.
- Current diagnostic methods may have limitations in sensitivity and specificity.
Purpose of the Study:
- To develop and evaluate novel long-circulating and pH-sensitive liposomes labeled with technetium-99m and ceftizoxime (SpHL-(99m)Tc-CF).
- To assess the potential of SpHL-(99m)Tc-CF for the diagnosis of osteomyelitis foci.
Main Methods:
- Development of long-circulating, pH-sensitive liposomes encapsulating ceftizoxime.
- Radiolabeling of liposomes with technetium-99m.
- Biodistribution studies and scintigraphic imaging in rats with and without bone infection.
Main Results:
- SpHL-(99m)Tc-CF demonstrated high accumulation in infectious foci.
- High target-to-non-target ratios were observed in infected areas.
- Scintigraphic images clearly visualized the bone infection sites.
Conclusions:
- SpHL-(99m)Tc-CF shows significant potential as an imaging agent for diagnosing osteomyelitis.
- The developed liposomal formulation enhances diagnostic capabilities for bone infections.
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