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Updated: May 21, 2026

Visualization of Organelles In Situ by Cryo-STEM Tomography
Published on: June 23, 2023
Seeing the atoms more clearly: STEM imaging from the Crewe era to today
1Oak Ridge National Laboratory, Materials Science and Technology Division, PO Box 2008, Oak Ridge, TN 37831-6071, United States. pennycooksj@ornl.gov
Abstract:
This review covers the development of scanning transmission electron microscopy from the innovations of Albert Crewe to the two-dimensional spectrum imaging in the era of aberration correction. It traces the key events along the path, the first atomic resolution Z-contrast imaging of individual atoms, the realization of incoherent imaging in crystals and the role of dynamical diffraction, simultaneous, atomic resolution electron energy loss spectroscopy, and finally the tremendous impact of the successful correction of lens aberrations, not just in terms of resolution but also in single atom sensitivity.
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