Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Peroxisomes

Peroxisomes

Peroxisomes are specialized organelles present in fungi, plant, and animal cells. It can vary in number, size, morphology, and activity depending on the type of tissue and the nutritional state of the cell. For example, cells with active lipid metabolism, such as adipocytes, neurons, and hepatocytes, have more peroxisomes than other cells in the body. Besides their primary role in breaking down complex organic molecules, peroxisomes can also synthesize specific macromolecules and participate in...

Peroxisomes and Mitochondria

Peroxisomes and Mitochondria

Peroxisomes and mitochondria are two important oxygen-utilizing organelles in eukaryotic cells. Mitochondria carry out cellular respiration—the process that converts energy from food into ATP. Peroxisomes carry out a variety of functions, primarily breaking down different substances, such as fatty acids.
The peroxisome is a single membrane-bound cellular organelle that can perform several different functions, including lipid metabolism and chemical detoxification. The enzymes within peroxisomes...

Targeted Cancer Therapies

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against specific...

Electron Transport Chain: Complex I and II

Electron Transport Chain: Complex I and II

The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...

Protein Import into the Peroxisomes

Protein Import into the Peroxisomes

Cells contain membrane-bound organelles called peroxisomes that oxidize organic molecules by transferring hydrogen atoms to oxygen, producing hydrogen peroxide. Peroxisomes enzymatically convert the released hydrogen peroxide into water and oxygen.
Peroxisomal Protein Import:
Peroxisomes lack the genetic machinery required to code for their own proteins. Hence, most peroxisomal membrane, lumenal and transmembrane proteins are synthesized in the cytoplasm or ER and transported to the peroxisome...

Redox Reactions

Redox Reactions

Oxidation-reduction or redox reactions involve the transfer of electrons from one molecule or atom to another. When an atom gains an electron, another atom must lose an electron, meaning oxidation and reduction must occur together. Since the redox occurs in pairs, the atom that gets oxidized is also called the reducing agent or reductant, and the atom that is reduced is also called the oxidizing agent or oxidant. A straightforward way to remember the definitions of oxidation and reduction is...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Ocular manifestations of patients with extranodal NK/T-cell lymphoma.

Eye (London, England)·2025

Same author

Rapid regulations of metabolic reactions in Escherichia coli via light-responsive enzyme redistribution.

Biotechnology journal·2022

Same author

Furin extracellularly cleaves secreted PTENα/β to generate C-terminal fragment with a tumor-suppressive role.

Cell death & disease·2022

Same author

Effects of HIF-1α, hepcidin and PTH on RankL in patients with chronic kidney disease in different stages.

Transplant immunology·2022

Same author

The phosphatase PTEN links platelets with immune regulatory functions of mouse T follicular helper cells.

Nature communications·2022

Same author

Effective production of Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) by engineered Halomonas bluephagenesis grown on glucose and 1,4-Butanediol.

Bioresource technology·2022

Search research articles

Related Experiment Video

Updated: May 21, 2026

Flow Cytometric Analysis of Mitochondrial Reactive Oxygen Species in Murine Hematopoietic Stem and Progenitor Cells and MLL-AF9 Driven Leukemia

Flow Cytometric Analysis of Mitochondrial Reactive Oxygen Species in Murine Hematopoietic Stem and Progenitor Cells and MLL-AF9 Driven Leukemia

Published on: September 5, 2019

Targeting peroxiredoxins against leukemia.

Chuan-Xu Liu¹, Hu-Chen Zhou, Qian-Qian Yin

¹Department of Pathophysiology, Shanghai Univeristies E-Institute for Chemical Biology, Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.

Experimental Cell Research

|June 26, 2012

Summary

Adenanthin is a natural molecule that inhibits peroxidase activities of Peroxiredoxins (Prx) I and II. This discovery offers a new therapeutic strategy for acute myeloid leukemia by inducing cell differentiation.

More Related Videos

Fast and Specific Assessment of the Halogenating Peroxidase Activity in Leukocyte-enriched Blood Samples

Fast and Specific Assessment of the Halogenating Peroxidase Activity in Leukocyte-enriched Blood Samples

Published on: July 28, 2016

Pre-clinical Evaluation of Tyrosine Kinase Inhibitors for Treatment of Acute Leukemia

Pre-clinical Evaluation of Tyrosine Kinase Inhibitors for Treatment of Acute Leukemia

Published on: September 18, 2013

Related Experiment Videos

Last Updated: May 21, 2026

Flow Cytometric Analysis of Mitochondrial Reactive Oxygen Species in Murine Hematopoietic Stem and Progenitor Cells and MLL-AF9 Driven Leukemia

Flow Cytometric Analysis of Mitochondrial Reactive Oxygen Species in Murine Hematopoietic Stem and Progenitor Cells and MLL-AF9 Driven Leukemia

Published on: September 5, 2019

Fast and Specific Assessment of the Halogenating Peroxidase Activity in Leukocyte-enriched Blood Samples

Fast and Specific Assessment of the Halogenating Peroxidase Activity in Leukocyte-enriched Blood Samples

Published on: July 28, 2016

Pre-clinical Evaluation of Tyrosine Kinase Inhibitors for Treatment of Acute Leukemia

Pre-clinical Evaluation of Tyrosine Kinase Inhibitors for Treatment of Acute Leukemia

Published on: September 18, 2013

Area of Science:

Biochemistry
Cell Biology
Oncology

Background:

Peroxiredoxins (Prx) regulate cellular reactive oxygen species (ROS), impacting signaling and disease pathogenesis.
Redox homeostasis is a key therapeutic target for diseases, including cancers.

Purpose of the Study:

To review recent discoveries on adenanthin as a novel therapeutic agent.
To highlight adenanthin's specific targeting of Peroxiredoxin I and II (Prx I/II) resolving cysteines.
To explore adenanthin's role in inducing differentiation of acute myeloid leukemia (AML) cells.

Main Methods:

Literature review of recent discoveries on adenanthin and Prx.
Analysis of adenanthin's mechanism of action on Prx I/II peroxidase activity.
Evaluation of adenanthin's effects on AML cell differentiation in vitro and in vivo.

Main Results:

Adenanthin identified as the first natural molecule to specifically inhibit Prx I and Prx II.
Adenanthin effectively targets the resolving cysteines of Prx I/II, reducing their peroxidase activity.
Adenanthin demonstrated efficacy in inducing differentiation of acute myeloid leukemic cells both in vitro and in vivo.

Conclusions:

Adenanthin represents a promising natural therapeutic candidate for targeting Prx-mediated redox signaling.
Inhibition of Prx activity by adenanthin offers a novel strategy for treating acute myeloid leukemia.
Adenanthin's ability to induce differentiation provides a potential mechanism for its anti-leukemic effects.