Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Surface Active Agents01:27

Surface Active Agents

Surfactants, named for their behavior at interfaces, positively adsorb at the interfaces of two phases, reducing interfacial tension. Their versatility as emulsifiers, detergents, and foaming agents stems from this ability. Surfactants, often termed amphiphiles, share the property of amphipathy, with molecules having both hydrophilic and hydrophobic portions. The hydrophilic part is called the head, and the hydrophobic part, including an elongated alkyl substituent, forms the tail.Surfactants...
Micelles01:30

Micelles

Micelle formation is an intricate process that hinges on the properties of amphiphilic or amphipathic molecules and the conditions of the system in which they are found. Amphiphilic molecules, which have both hydrophilic (water-attracting) and hydrophobic (water-repelling) parts, play a critical role in this process.In aqueous environments, these molecules arrange themselves such that their hydrophilic heads are turned towards the water phase, while their hydrophobic tails are oriented away...
Biosynthesis of Lipids01:29

Biosynthesis of Lipids

Microbial membranes exhibit remarkable diversity in lipid composition, reflecting evolutionary adaptations to various environmental conditions. The three domains of life—Bacteria, Archaea, and Eukarya—synthesize membrane lipids through distinct biosynthetic pathways, leading to fundamental structural differences that impact membrane stability, function, and adaptability.Fatty Acid-Based Lipids in Bacteria and EukaryaBacteria and eukaryotes share a common fatty acid biosynthesis pathway, which...
Breathing01:05

Breathing

The process of breathing, inhaling and exhaling, involves the coordinated movement of the chest wall, the lungs, and the muscles that move them. Two muscle groups with important roles in breathing are the diaphragm, located directly below the lungs, and the intercostal muscles, which lie between the ribs. When the diaphragm contracts, it moves downward, increasing the volume of the thoracic cavity and creating more room for the lungs to expand. When the intercostal muscles contract, the ribs...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Decoding cultured meat manufacturing: a full process model to identify scale-up bottlenecks.

Frontiers in nutrition·2026
Same author

Acetate as alternative carbon source for production of mono- and di-rhamnolipids in Pseudomonas putida KT2440.

Microbial cell factories·2026
Same author

Exploring the metabolic burden of surfactin biosynthesis and the metabolic costs of srfA operon expression in Bacillus subtilis.

Microbial cell factories·2026
Same author

Exploiting the ability of Bacillus subtilis to synthetize surfactin under oxygen limitation.

Applied microbiology and biotechnology·2026
Same author

Design and Characterization of a 3D-Printable Membrane Aeration Module for Small-Scale Bioprocess Prototyping.

Engineering in life sciences·2026
Same author

Bioreactor Design and Engineering for Cultivated Meat Manufacturing.

Advances in biochemical engineering/biotechnology·2026

Related Experiment Video

Updated: May 21, 2026

Enhanced Oil Recovery using a Combination of Biosurfactants
13:19

Enhanced Oil Recovery using a Combination of Biosurfactants

Published on: June 3, 2022

Rhamnolipids--next generation surfactants?

Markus Michael Müller1, Johannes H Kügler, Marius Henkel

  • 1Institute of Process Engineering and Life Sciences, Section II: Technical Biology, Karlsruhe Institute of Technology-KIT, Engler-Bunte-Ring 1, D-76131 Karlsruhe, Germany. arkusmichaelmueller@web.de

Journal of Biotechnology
|June 26, 2012
PubMed
Summary

Rhamnolipids show promise as next-generation biosurfactants, but optimizing their production using sustainable, non-food resources requires further research into cellular regulation and metabolic engineering.

More Related Videos

Detection and Quantification of Mono-Rhamnolipids and Di-Rhamnolipids Produced by Pseudomonas aeruginosa
04:37

Detection and Quantification of Mono-Rhamnolipids and Di-Rhamnolipids Produced by Pseudomonas aeruginosa

Published on: March 29, 2024

Studying Surfactant Effects on Hydrate Crystallization at Oil-Water Interfaces Using a Low-Cost Integrated Modular Peltier Device
06:31

Studying Surfactant Effects on Hydrate Crystallization at Oil-Water Interfaces Using a Low-Cost Integrated Modular Peltier Device

Published on: March 18, 2020

Related Experiment Videos

Last Updated: May 21, 2026

Enhanced Oil Recovery using a Combination of Biosurfactants
13:19

Enhanced Oil Recovery using a Combination of Biosurfactants

Published on: June 3, 2022

Detection and Quantification of Mono-Rhamnolipids and Di-Rhamnolipids Produced by Pseudomonas aeruginosa
04:37

Detection and Quantification of Mono-Rhamnolipids and Di-Rhamnolipids Produced by Pseudomonas aeruginosa

Published on: March 29, 2024

Studying Surfactant Effects on Hydrate Crystallization at Oil-Water Interfaces Using a Low-Cost Integrated Modular Peltier Device
06:31

Studying Surfactant Effects on Hydrate Crystallization at Oil-Water Interfaces Using a Low-Cost Integrated Modular Peltier Device

Published on: March 18, 2020

Area of Science:

  • Biotechnology and Industrial Microbiology
  • Sustainable Chemistry
  • Biochemical Engineering

Background:

  • Increasing demand for bio-based chemicals drives innovation in the petrochemical industry.
  • Biosurfactants, like rhamnolipids, offer sustainable and biodegradable alternatives to petroleum-based products.
  • Current rhamnolipid production is limited by a lack of quantitative understanding for bioreactor cultivation and reliance on specific strains and feedstocks.

Purpose of the Study:

  • To review current approaches for optimizing rhamnolipid production.
  • To explore strategies for enhancing rhamnolipid product spectra.
  • To identify challenges and future directions for commercializing rhamnolipids.

Main Methods:

  • Review of optimization strategies for rhamnolipid production in Pseudomonas aeruginosa.
  • Examination of screening methods for novel, non-pathogenic rhamnolipid producers.
  • Discussion of recombinant production approaches and biocatalysis applications.

Main Results:

  • Rhamnolipids possess high potential as next-generation biosurfactants.
  • Quantitative understanding of biosynthesis regulation is crucial for efficient bioreactor cultivation.
  • Development of non-pathogenic strains and utilization of non-food renewable substrates are key for sustainable production.

Conclusions:

  • Significant obstacles remain in rhamnolipid production and commercialization.
  • A combination of X-omics strategies and metabolic engineering is needed for advancement.
  • Further research is required to achieve competitive productivity with sustainable feedstocks.