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Related Concept Videos

Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Protein-protein Interfaces02:04

Protein-protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...
Protein-Protein Interfaces02:04

Protein-Protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...

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Mapping Dysfunctional Protein-Protein Interactions in Disease
09:39

Mapping Dysfunctional Protein-Protein Interactions in Disease

Published on: October 24, 2025

Rewiring the dynamic interactome.

Melissa J Davis1, Chang Jin Shin, Ning Jing

  • 1Institute for Molecular Bioscience, The University of Queensland, St Lucia, Queensland 4072, Australia.

Molecular Biosystems
|June 26, 2012
PubMed
Summary
This summary is machine-generated.

Transcriptomics reveals that human genes produce diverse transcripts, altering protein interactions. This variation significantly impacts the human protein-interaction network, known as the interactome.

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Area of Science:

  • Molecular Biology
  • Systems Biology
  • Genomics

Background:

  • Eukaryotic genes generate multiple transcripts, leading to diverse protein isoforms.
  • Protein-protein interactions are crucial in biological systems but often studied at the gene level.
  • Transcript and protein isoform diversity can significantly impact cellular functions.

Purpose of the Study:

  • To investigate the impact of transcript and isoform diversity on protein interaction networks.
  • To identify variations in protein interaction domains due to alternative splicing.
  • To characterize how transcriptional variation rewires the human interactome.

Main Methods:

  • Utilized full-length human cDNAs to analyze protein-coding transcriptional output.
  • Identified variations in the inclusion of protein interaction domains across transcripts.
  • Mapped transcriptomic data to high-quality protein interaction datasets.

Main Results:

  • Found significant variation in protein interaction domain inclusion in nearly 20% of human genes.
  • Demonstrated that transcriptional variation can alter network connectivity.
  • Showed that alternative splicing can significantly rewire the human interactome.

Conclusions:

  • Transcriptional and isoform diversity play a critical role in shaping the human interactome.
  • Understanding transcript variation is essential for a comprehensive view of biological networks.
  • This study highlights the dynamic nature of protein interactions driven by gene expression complexity.