Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Histone Variants at the Centromere02:30

Histone Variants at the Centromere

Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3 variants are also...
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
Catenins01:23

Catenins

Catenins are characterized by multiple binding domains and dynamic structures that allow them to function as linker proteins in cell junction complexes. All catenins, except α-catenin, contain a characteristic protein sequence called the armadillo repeat and are therefore also called armadillo proteins.
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the adherens...
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)01:15

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT) is an advanced Nuclear Magnetic Resonance (NMR) technique specifically designed to detect and enhance the signals of low-abundance nuclei, such as carbon-13 and nitrogen-15, in small molecules. The fundamental principle behind INEPT is the transfer of polarization from a more abundant and highly polarizable nucleus, typically hydrogen-1, to the low-abundance nucleus of interest. This process effectively boosts the NMR signal of the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Culture-enriched metagenomic sequencing reveals within-patient diversity and transmission of vancomycin-resistant <i>Enterococcus faecium</i>.

Microbial genomics·2026
Same author

Transcriptomic and proteomic responses to gas vesicle collapse in native and engineered bacterial systems.

bioRxiv : the preprint server for biology·2026
Same author

Case Commentary: When one target is not enough-PBP3 insertions and target redundancy in <i>Escherichia coli</i>.

Antimicrobial agents and chemotherapy·2026
Same author

Culture-enriched metagenomic sequencing reveals within-patient diversity and transmission of vancomycin-resistant <i>Enterococcus faecium</i>.

medRxiv : the preprint server for health sciences·2026
Same author

Human CRAMP1 specifically promotes the expression of histone H1 genes.

EMBO reports·2026
Same author

Rate-limiting enzymes in nucleotide metabolism synchronize nucleotide biosynthesis and chromatin formation.

Molecular cell·2025

Related Experiment Video

Updated: May 21, 2026

Generation of Centromere-Associated Protein-E CENP-E-/- Knockout Cell Lines using the CRISPR/Cas9 System
11:49

Generation of Centromere-Associated Protein-E CENP-E-/- Knockout Cell Lines using the CRISPR/Cas9 System

Published on: June 23, 2023

Putting CENP-A in its place.

Madison E Stellfox1, Aaron O Bailey, Daniel R Foltz

  • 1Department of Biochemistry and Molecular Genetics, University of Virginia Medical School, PO Box 800733, Charlottesville, VA 22908, USA.

Cellular and Molecular Life Sciences : CMLS
|June 26, 2012
PubMed
Summary

Centromere identity relies on the epigenetic mark CENP-A (centromere protein A). New CENP-A nucleosomes are deposited during G1, guided by the Mis18 complex and HJURP, ensuring faithful chromosome segregation.

More Related Videos

Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A (EYFP-CENP-A)
09:02

Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A (EYFP-CENP-A)

Published on: June 10, 2020

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins
05:35

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

Published on: March 3, 2016

Related Experiment Videos

Last Updated: May 21, 2026

Generation of Centromere-Associated Protein-E CENP-E-/- Knockout Cell Lines using the CRISPR/Cas9 System
11:49

Generation of Centromere-Associated Protein-E CENP-E-/- Knockout Cell Lines using the CRISPR/Cas9 System

Published on: June 23, 2023

Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A (EYFP-CENP-A)
09:02

Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A (EYFP-CENP-A)

Published on: June 10, 2020

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins
05:35

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

Published on: March 3, 2016

Area of Science:

  • Cell Biology
  • Epigenetics
  • Molecular Biology

Background:

  • The centromere is crucial for accurate chromosome segregation during mitosis.
  • Centromeric identity is epigenetically determined by CENP-A nucleosomes.
  • Maintaining centromeric function requires cell-cycle-dependent CENP-A deposition.

Purpose of the Study:

  • To review the unique characteristics of centromeric chromatin.
  • To elucidate the mechanism of CENP-A nucleosome deposition.
  • To highlight recent discoveries in the regulation of CENP-A assembly.

Main Methods:

  • Literature review of centromeric chromatin and CENP-A deposition.
  • Analysis of the roles of the Mis18 complex and HJURP.
  • Discussion of temporal and spatial control factors.

Main Results:

  • CENP-A nucleosomes serve as the epigenetic mark for active centromeres.
  • New CENP-A nucleosomes are deposited in early G1, following S-phase distribution.
  • The Mis18 complex and HJURP are essential for CENP-A assembly.

Conclusions:

  • Faithful chromosome segregation depends on the precise epigenetic specification of centromeres.
  • Understanding CENP-A deposition mechanisms is key to centromere maintenance.
  • Ongoing research is uncovering regulatory factors for CENP-A assembly.