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Related Concept Videos

Dialysis01:15

Dialysis

Dialysis is a diffusion-based purification process that separates analyte molecules from a complex matrix. This is accomplished by allowing molecules in the solution to pass through a semipermeable membrane into a liquid on the other side. The membrane is usually made of cellulose acetate or cellulose nitrate, and the second liquid must be miscible with the solution. Ions (e.g., chloride or sodium) or organic molecules (e.g., glucose) can pass through the membrane pores, which generally have...
Protein-Drug Binding: Determination Methods01:22

Protein-Drug Binding: Determination Methods

Determining protein-drug binding can be achieved through indirect and direct methods, each providing valuable insights into the interaction between proteins and drugs.
Indirect methods involve isolating the bound drug from its free form in biological samples such as blood, serum, or plasma. These techniques aim to measure the percentage of drugs bound to proteins. Equilibrium dialysis is a commonly used method where the free drug concentration at equilibrium is measured by separating the bound...

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Related Experiment Video

Updated: May 21, 2026

Custom-made Microdialysis Probe Design
05:38

Custom-made Microdialysis Probe Design

Published on: July 21, 2015

Approach to reduce the non-specific binding in microdialysis.

Ramakrishna Nirogi1, Vishwottam Kandikere, Gopinadh Bhyrapuneni

  • 1Discovery Research, Suven Life Sciences Ltd, Serene Chambers, Road 5 Avenue 7, Banjara Hills, Hyderabad, India.

Journal of Neuroscience Methods
|June 27, 2012
PubMed
Summary

This study presents a systematic approach to predict and reduce non-specific binding in microdialysis, improving the accuracy of brain pharmacokinetic measurements for drug discovery. This method enhances the reliability of monitoring drug concentrations in the brain.

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Published on: September 26, 2018

Area of Science:

  • Pharmacology
  • Neuroscience
  • Analytical Chemistry

Background:

  • Accurate measurement of unbound drug concentrations at the biophase is crucial in drug discovery.
  • Microdialysis is a key technique for in vivo monitoring of endogenous and exogenous compounds, particularly in brain pharmacokinetics.
  • Non-specific binding of test compounds to microdialysis equipment can compromise the accuracy of extracellular concentration determination.

Purpose of the Study:

  • To develop and demonstrate a systematic approach for predicting and reducing non-specific binding in microdialysis.
  • To adapt study conditions based on compound adsorption properties to minimize binding.
  • To enhance the reliability of microdialysis for brain pharmacokinetic evaluations.

Main Methods:

  • In vitro measurement of non-specific binding to microdialysis tubing for diverse compounds.
  • Evaluation of in vitro probe extraction efficiency using gain and loss methods.
  • In vivo assessment of probe extraction efficiency and brain pharmacokinetics in rats.

Main Results:

  • Developed methods to predict and quantify non-specific binding to microdialysis tubing and probe membranes.
  • Adapted experimental conditions based on in vitro binding characteristics.
  • Successfully evaluated brain pharmacokinetics, demonstrating reduced impact of non-specific binding.

Conclusions:

  • The described stepwise approach effectively evaluates and minimizes non-specific binding in microdialysis.
  • This methodology strengthens the application of microdialysis for accurate brain pharmacokinetic studies.
  • Improved accuracy in measuring unbound drug concentrations supports robust drug discovery efforts.