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Updated: May 21, 2026
Quantitative Imaging of Lineage-specific Toll-like Receptor-mediated Signaling in Monocytes and Dendritic Cells from Small Samples of Human Blood
Published on: April 16, 2012
Age-related decrease of the LAMP-2 gene expression in human leukocytes
Jian Huang1, Jing Xu, Shuchao Pang
1Shandong Provincial Key Laboratory of Cardiac Disease Diagnosis and Treatment, Jining Medical College Affiliated Hospital, Jining Medical College, Jining, Shandong 272029, China.
Objective:
Autophagy is a highly conserved degradation pathway in cells, which has been involved in many physiological processes and implicated in human age-related diseases. However, autophagy activities have not been systemically investigated with human tissues and cells.
Methods:
Lysosomal associated membrane protein-2 (LAMP-2) protein is critical for autophagy and chaperone-mediated autophagy. We examined LAMP-2 gene expression and protein levels in the peripheral leukocytes from healthy subjects over 40 years old.
Results:
Compared to those in group of 40-44 years, the LAMP-2 transcript and protein levels in groups of 65-69 (P<0.01) and over 70 years (P<0.001) were significantly decreased. No significant difference in LAMP-2 transcript and protein levels were observed between male and female groups.
Conclusions:
Our data revealed that there was a progressive and age-related decrease of the LAMP-2 gene expression in the peripheral leukocytes of healthy subjects, indicating a trend of decreasing autophagy activities with aging.
