Related Experiment Videos
Replication of an entomopoxvirus in two lepidopteran cell lines
1Faculty of Agriculture, Tokyo University of Agriculture and Technology, Japan.
Abstract:
Pseudaletia separata entomopoxvirus replicated in two lepidopteran cell lines, SIE-MSH-805-F and BM-N. Microscopic examination, and the virus passage tests, of infected cultures indicated that the virus replicated more readily in the former cell line. Virus release by exocytosis occurred in both cell lines. A sequence of virus morphogenesis in the cultured cells was described, based on electron microscopic observations of thin sections. The nucleus of infected cells contained spherical inclusions, and the cytoplasm contained virions, immature virus forms, spheroids, and spindles. A portion of the virions in the cytoplasm was occluded within spheroids, which were often associated with crystallogenic matrix. Virions acquired a coat prior to their occlusion.
Insights
Pseudaletia separata entomopoxvirus replicates in lepidopteran cell lines, with better replication in SIE-MSH-805-F. Virus release occurs via exocytosis, and morphogenesis involves inclusions and virions within spheroids.
Area of Science:
- Virology
- Insect Pathology
- Cell Biology
Background:
- Entomopoxviruses are significant pathogens of insects.
- Understanding virus-host interactions is crucial for developing biocontrol strategies.
- Pseudaletia separata is an important agricultural pest.
Purpose of the Study:
- To investigate the replication and morphogenesis of Pseudaletia separata entomopoxvirus in cultured lepidopteran cells.
- To compare the replication efficiency in two different cell lines.
- To describe the sequence of virus development within infected cells.
Main Methods:
- Infection of two lepidopteran cell lines (SIE-MSH-805-F and BM-N) with Pseudaletia separata entomopoxvirus.
- Monitoring viral replication through microscopic examination and virus passage tests.
- Electron microscopy of thin sections to observe virus morphogenesis.
Main Results:
- Pseudaletia separata entomopoxvirus replicated in both cell lines, with higher efficiency in SIE-MSH-805-F.
- Virus release was observed via exocytosis in both cell lines.
- Electron microscopy revealed distinct stages of virus morphogenesis, including nuclear inclusions and cytoplasmic virions, spheroids, and spindles, with virion occlusion within spheroids.
Conclusions:
- Lepidopteran cell lines provide a suitable system for studying Pseudaletia separata entomopoxvirus replication.
- SIE-MSH-805-F is a more permissive cell line for this virus.
- The study elucidated key aspects of entomopoxvirus morphogenesis in vitro.