Separation methods for captopril in pharmaceuticals and biological fluids
Related Experiment Video
Updated: May 21, 2026

Evaluation of Drug Sorption to PVC- and Non-PVC-based Tubes in Administration Sets Using a Pump
Published on: March 11, 2017
Fotouh R Mansour1, Neil D Danielson
1Department of Chemistry and Biochemistry, Miami University, Oxford, OH 45056, USA.
Abstract:
Captopril (CAP) is an orally active angiotensin-converting enzyme (ACE) inhibitor and has been widely used for management of hypertension and congestive heart failure. CAP lacks an aromatic chromophore required for facile direct UV detection and also has two chiral centers. These factors can render the determination of CAP in complex matrices challenging. This review covers more than 20 years of analytical research on this drug, focusing mainly on pharmaceutical and biological applications. The primary separation techniques discussed are gas chromatography, liquid chromatography, and capillary electrophoresis. The structures of the CAP derivatizing agents as well as a table summarizing various HPLC methods are provided. A discussion of key recent chromatographic and electrophoretic methods for other ACE inhibitors is also present.
Related Concept Videos
Capillary Electrophoresis: Applications
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Electrophoresis: Overview
There...
High-Performance Liquid Chromatography: Elution Process
Capillary Electrophoresis: Instrumentation
Drug Elimination by Renal Route: Tubular Secretion
Extracorporeal Removal of Drugs: Hemoperfusion and Hemofiltration

