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Related Concept Videos

Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
Size-Exclusion Chromatography01:08

Size-Exclusion Chromatography

In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...
High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...

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Related Experiment Video

Updated: May 21, 2026

Ion Exchange Chromatography (IEX) Coupled to Multi-angle Light Scattering (MALS) for Protein Separation and Characterization
10:41

Ion Exchange Chromatography (IEX) Coupled to Multi-angle Light Scattering (MALS) for Protein Separation and Characterization

Published on: April 5, 2019

Chiral separation using capillary electromigration techniques based on ligand exchange principle.

Haizhi Zhang1, Li Qi, Lanqun Mao

  • 1Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing, China.

Journal of Separation Science
|June 27, 2012
PubMed
Summary
This summary is machine-generated.

This review explores chiral ligand-exchange (CLE) methods in capillary electrophoresis (CE) and capillary electrochromatography (CEC). It highlights CLE applications in life science and food analysis using various separation modes and novel columns.

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Capillary Electrophoresis Separation of Monoclonal Antibody Isoforms Using a Neutral Capillary
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Capillary Electrophoresis Separation of Monoclonal Antibody Isoforms Using a Neutral Capillary

Published on: January 16, 2017

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Last Updated: May 21, 2026

Ion Exchange Chromatography (IEX) Coupled to Multi-angle Light Scattering (MALS) for Protein Separation and Characterization
10:41

Ion Exchange Chromatography (IEX) Coupled to Multi-angle Light Scattering (MALS) for Protein Separation and Characterization

Published on: April 5, 2019

Capillary Electrophoresis Separation of Monoclonal Antibody Isoforms Using a Neutral Capillary
14:53

Capillary Electrophoresis Separation of Monoclonal Antibody Isoforms Using a Neutral Capillary

Published on: January 16, 2017

Area of Science:

  • Analytical Chemistry
  • Separation Science

Background:

  • Diverse chiral separation methods have evolved from fundamental principles over the past two decades.
  • Chiral ligand-exchange (CLE) is a key principle driving advancements in chiral separations.

Purpose of the Study:

  • To review the application of chiral ligand-exchange (CLE) in capillary electromigration techniques.
  • To discuss various CLE separation modes and novel column technologies for chiral analysis.

Main Methods:

  • Focus on chiral ligand-exchange capillary electrophoresis (CLE-CZE) using various central ions (e.g., Cu(II), Zn(II), borate).
  • Exploration of CLE micellar electrokinetic chromatography (CLE-MEKC) with different surfactants.
  • Description of novel packed and monolithic capillary columns for CLE-CEC.

Main Results:

  • CLE-CZE effectively separates chiral compounds using diverse metal ions and borate.
  • CLE-MEKC utilizes surfactants for enhanced chiral recognition.
  • Novel capillary columns significantly advance CLE-CEC for chiral separations.

Conclusions:

  • Chiral ligand-exchange is a versatile principle for capillary electrophoresis and electrochromatography.
  • Advanced CLE techniques and column designs offer effective chiral separation solutions.
  • These methods are valuable for applications in life sciences and food analysis.