Selenium cannot substitute for sulfur in cell density-independent bioluminescence in Vibrio fischeri

It has been proposed that selenium, an element chemically similar to sulfur, can participate in some of the same biological pathways as sulfur, although only a few studies have been confirmed this. In this study, we investigated the relationship between selenium and sulfur-dependent luminescence in Vibrio fischeri. The luminescence of V. fischeri was induced by the addition of sulfur-containing compounds such as Na₂SO₄ and L-cystine, and their luminescence was suppressed, in a dose-dependent manner, by the addition of the selenium-containing compounds Na₂SeO₄ and L-selenocystine. Since the viability of V. fischeri was not affected by the addition of low concentration of selenium-containing compounds, the decrease in luminescence intensity cannot be explained by cell death. Kinetic analysis performed using Lineweaver-Burk plots demonstrate that Na₂SeO₄ and L-selenocystine act as competitive suppressors in inorganic sulfur (Na₂SeO₄)-dependent luminescence. In contrast, these selenium-containing compounds act as uncompetitive suppressors in organic sulfur (L-cystine)-dependent luminescence.